摘要
从青藏高原牦牛粪中分离出1株可降解毒死蜱的细菌,经形态和16SrDNA序列分析,鉴定其为鞘氨醇单胞菌属(Sphingomonas sp.)细菌。对其进行重离子诱变并筛选出菌株Sph-25-T1,该菌株对100mg.L-1毒死蜱的72h降解率达98.44%,比出发菌株提高27.37%。对Sph-25-T1进行传代培养,第5代时其对毒死蜱的降解率依然保持在90%以上。以喷洒毒死蜱1周后的不同土壤浸出液或蔬菜叶片做培养基时,Sph-25-T1对毒死蜱的降解率达到29.68%～78.40%。
A strain with the ability of chlorpyrifos degradation was isolated from the yak dung on Qinghai-Tibet Plateau,and identified as Sphingomonas sp.based on the phenotype and 16S rDNA sequence analysis.In present study,we used heavy-ion beam irradiation to mutagenize the strain and screened a mutant named Sph-25-T1.The experiment results showed that the strain Sph-25-T1 strongly degraded chlorpyrifos added in the medium,the degradation rate of 100 mg·L-1 chlorpyrifos added in medium was by 98.44% after 72h and 27.37% higher than that of the original strain.The strain Sph-25-T1 also degraded chlorpyrifos by 90% above after five generations cultivation.In the mediums of soil extract and vegetable leaves after spraying chlorpyrifos for one week,the strain Sph-25-T1 had the degradation rate of chlorpyrifos by 29.68%-78.40% after 84 h.
出处
《西北农业学报》
CAS
CSCD
北大核心
2011年第12期135-139,共5页
Acta Agriculturae Boreali-occidentalis Sinica
基金
国家自然科学基金项目(30960063)
关键词
重离子
生物降解
毒死蜱
鞘氨醇单胞菌
Heavy-ion beam irradiation
Biodegradation
Chlorpyrifos
Sphingomonas sp.
作者简介
第一作者：台喜生，男，硕士研究生，研究方向为环境污染物的微生物降解和修复。E-mail：leafturnred@163．com通讯作者：李师翁。Email：lishweng@mail．lzjtu．cn.
