摘要
采用蛭石栽培试验,在100mmol.L-1 NaCl胁迫下,对耐盐性不同的两个菜用大豆品种种子膨大、抗氧化系统及渗透调节物质进行了研究。结果显示:(1)耐盐品种‘绿领特早’种子干质量及大小的增加在NaCl胁迫15d时未受显著影响,而盐敏感品种‘理想高产95-1’种子的膨大受到了显著抑制。(2)NaCl胁迫导致菜用大豆种子超氧阴离子(O2.-)、过氧化氢(H2O2)及丙二醛(MDA)的过量积累,但‘绿领特早’种子的相对积累量均低于‘理想高产95-1’。(3)与‘理想高产95-1’相比,‘绿领特早’在NaCl胁迫期间维持了相对较高的超氧化物歧化酶(SOD)、抗坏血酸过氧化物酶(APX)及谷胱甘肽还原酶(GR)活性,以及较高的抗坏血酸(AsA)、可溶性蛋白和可溶性糖含量;胁迫中期维持了相对较高的过氧化物酶(POD)活性,胁迫后期维持了相对较高的过氧化氢酶(CAT)活性;胁迫前中期维持了相对较高的谷胱甘肽(GSH)含量。研究发现,‘绿领特早’种子在NaCl胁迫期间能够保持较高且协调平衡的保护酶SOD、POD、CAT活性和较高的AsA-GSH循环效率,同时维持了较强的渗透调节能力,有效抑制了O2.-和H2O2的过量积累,进而有效降低了细胞膜质过氧化程度,这可能是其耐盐性较强的重要原因之一。
With vermiculite culture,seed hlhng,and their ant l/ NaCI stress The re- two vegetable soybean cultivars with different salt tolerance ＇ sults showed that： （1） Seed enlargement was normal for salt resistant cultivar ＇ Liilingtezao but seed enlargement was inhibited for salt susceptible cultivar ＇ Lixianggaochan 95-1＇ under NaC1 stress for 15 h. （Z）O2^- producting rate, H2 02 and malondialdehyde （MDA） contents in seeds of two cultivars were significantly increased un- der NaC1 stress,while the increasing amount of salt resistant cultivar Lulmgteza was lower than those of salt susceptible cultivar ＇ Lixianggaochan 95-1＇. （3）During the period of NaC1 stress,the activities of su- peroxide dismutase （SOD）,ascorbate peroxidase （APX） and glutathione reductase （GR）,the contents of aseorbic acid （AsA）, soluble protein and soluble sugar of ＇L01ingtezao＇ were relatively higher compared with those of ＇Lixianggaochan 95-1＇ ,and its activities of peroxidase （POD） and catalase （CAT）,content of reduced glutathione （GSH） maintained relatively higher values in the intermediate, later, early and interme- diate periods, respectively, under NaC1 stress compared with those of ‘ Lixianggaocban 95-1 ＇. These results indicated that ＇Ltilingtezao ＇ could maintain higher and mutually concordant activities of SOD, POD and CAT,maintain efficient metabolism of AsA-GSH cycle and stronger osmotic regulation ability. These re- sulted in the effective inhibition of lipid peroxidation, which might be one of the important reasons for stronger salt tolerance of ‘Lulingtezao’.
出处
《西北植物学报》
CAS
CSCD
北大核心
2012年第2期297-305,共9页
Acta Botanica Boreali-Occidentalia Sinica
基金
“十一五”国家高技术研究发展计划重大项目(2006AA10A110)
国家转基因生物新品种培育科技重大专项(2009ZX08004-011B)
内蒙古民族大学科研创新团队计划项目(NMD1003)
内蒙古民族大学博士启动基金项目(BS217)
作者简介
王聪（1968-），男，教授，博士，主要从事蔬菜生理和生物技术研究。E-mail：tongliaowangcon8：@163．com
通讯作者：朱月林，教授，博士生导师，主要从事蔬菜生理和生物技术研究。E-mail：ylzhu@njau．edu．cn
