摘要
目的:应用植物类药材通用条形码ITS2鉴定中药材灯心草。方法:对灯心草药材及其密切相关种进行PCR扩增和测序,运用CodonCode Aligner对所获ITS2序列进行拼接。应用MEGA5.0软件计算Kimura 2-parameter(K2P)遗传距离。采用相似性搜索法、最近距离法、PWG距离法及构建NJ(邻接)系统聚类树等方法进行鉴定分析。结果:灯心草药材种内K2P遗传距离在0～0.005,远远小于灯心草与其他密切相关种之间的K2P遗传距离(0.215～0.614);4种鉴定方法均表明ITS2序列可以有效区分灯心草与其密切相关种。结论:植物类药材通用条形码ITS2适用于鉴定中药材灯心草,进一步证明了ITS2对中药材的鉴定能力。
Objective： To identify Junci Medulla using the ITS2 barcode.Method： The ITS2 regions of Juncus effuses and its closely related species were PCR amplified and sequenced.Sequence assembly and consensus sequence generation were performed using the CodonCode Aligner.The Kimura 2-Parameter（K2P） distances were calculated using software MEGA 5.0.Identification analyses were performed using BLAST1,Nearest Distance,PWG Distance and neighbor-joining（NJ） methods.Result： The intra-specific genetic distances of J.effuses were ranged from 0 to 0.005,which were far lower than inter-specific genetic distances between J.effuses and its closely related species（0.215-0.614）.All the four methods showed that ITS2 could discriminate J.effuses from its closely related species correctly.Conclusion： The ITS2 region is an efficient barcode for authentication of Junci Medulla,and our study further confirmed the ability of ITS2 to identify traditional Chinese medicinal materials.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2012年第8期1097-1099,共3页
China Journal of Chinese Materia Medica
基金
高等学校博士学科点专项科研基金资助课题(20111106120029)
作者简介
庞晓慧，助理研究员，从事中药资源与中药分子鉴定研究，Tel：（010）62899729，E-mail：xhpang@implad．ac．cn
[通信作者]陈士林，博士生导师，从事中药资源学研究，Tel：（010）62899700，E—mail：slchen@implad．ac．cn
