摘要
目的通过使用盐酸异丙肾上腺素(ISO)制备心肌损伤模型,探讨磷脂酰肌醇3激酶/丝氨酸苏氨酸蛋白激酶(PI3K/AKT)信号通路是否参与心肌损伤的发病机制。方法 20只雄性Wistar大鼠随机分成心肌损伤模型组(ISO组)和对照组。ISO组大鼠每天背部皮下注射ISO 20 mg.kg-1.d-1,对照组大鼠每天注射等量9 g.L-1盐水,均连续注射7 d。采用全自动生化检测仪检测2组大鼠血浆中乳酸脱氢酶(LDH)、肌酸激酶(CK)和α-羟丁酸脱氢酶(α-HBDH)水平。采用蛋白印迹法定量检测大鼠心肌组织中PI3K、p85-PI3K、p55-PI3K、AKT及p-AKT蛋白表达。结果与对照组比较,ISO组大鼠血浆LDH、CK及α-HBDH水平均显著上升,差异有统计学意义(Pa<0.05),证明采用ISO建模成功;与对照组比较,ISO组大鼠心肌组织中PI3K和AKT蛋白水平未见明显变化,但其磷酸化p85-PI3K、p55-PI3K和p-AKT蛋白水平均显著上调,差异有统计学意义(Pa<0.05)。结论在ISO所致的心肌损伤中,PI3K和AKT的磷酸化水平上调,PI3K/AKT信号通路参与ISO所致心肌损伤的发病机制。
Objective To explore whether the phosphoinositide 3 - kinase/serine - threonine protein kinase （PI3K/AKT） signaling pathway was related to the mechanism of isoproterenol（ISO） - induced myocardial injury. Methods Twenty male Wistar rats were randomly divided into ISO group and control group. In ISO group ,the rats were administered with ISO of 20 mg· kg^-1 · d^-1 via subcutaneous injection while in the control group ,the rats were administered with saline via subcutaneous injection. The duration of the above mentioned treatment was 7 days. The levels of lactate dehydrogenase （LDH） ,creatine kinase （CK） and alpha - hydroxybutyrate dehydrogenase （ α - HBDH） in plasma of rats in the 2 groups were detected by using automatic biochemical detector. PI3K,p85 - PI3K,p55 - PI3K,AKT and p - AKT pro- tein expressions in myocardial tissue were detected by using Western - blot method. Results Plasma LDH, CK and α - HBDH levels increased significantly in ISO group compared with control group（ Pa 〈 0.05 ） , which suggested that the myocardial injury model was made suc- cessfully. There were no statistically significant differences in PI3K and AKT protein expressions between ISO group and control group,while p85 - PI3 K, p55 - PI3 K and p - AKT protein expressions increased significantly in ISO group compared with control group （ Pa 〈 0.05 ）. Conclusions The phosphorylation levels of PI3K and AKT increase in rats with ISO - induced myocardial injury,and PI3K/AKT signaling pathway may be related to the mechanism of ISO - induced myocardial injury.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2012年第7期534-536,共3页
Journal of Applied Clinical Pediatrics
基金
国家自然科学基金(81070111)
作者简介
陈珊珊，女，硕士生，研究方向为心肺循环，电子信箱s.s_chen@yahoo．com．cn。
通讯作者：杜军保，男，主任医师，教授，博士学位，博士生导师，研究方向为心肺循环，电子信箱junbaodul@126．com。
