摘要
高效微生物菌系和适宜反应条件是纤维素生物转化的关键。试验以前期分离获得的纤维素降解菌为基础,研究了降解菌之间的协同效应和粗酶液性质。结果表明,降解菌之间存在协同效应,其中X2与X5混合培养产酶能力达到了0.66 U.mL-1,比单一降解菌酶活力提高了将近1倍;其粗酶液最适反应温度和pH值为25℃和5.0;金属离子络合剂EDTA对酶活力的抑制效果较为明显;Ca2+和Mn2+是粗酶液的激活剂,在研究浓度范围内能够显著提升酶活力。研究结果为纤维素的生物转化提供了重要种质资源和技术参数。
Efficient microbial strains and appropriate reaction conditions are the keys to the bio-transformation of cellulose.Based on cellulose-decomposing microorganisms isolated,we studied synergism of cellulose-decomposing microorganisms and properties of crude enzyme liquid.Results showed that synergism was significant between the degrading bacterium,enzyme activity that X2 and X5 mixed culture produced was up to 0.66 U·mL-1,which increased nearly 1 times than that of single-degrading bacteria.The optimum temperature and pH of crude enzyme were 25℃ and 5.0 respectively.The inhibitory effect of EDTA on the enzyme activity was more obvious.Ca2＋ and Mn2＋ were activators of the crude enzyme solution,which could significantly enhance the enzyme activity in the concentration range of the study.The results provided important germplasm resources and technical parameters for the bioconversion of cellulose.
出处
《浙江农业学报》
CSCD
北大核心
2012年第2期279-283,共5页
Acta Agriculturae Zhejiangensis
基金
黑龙江省教育厅科学技术研究项目(11551539)
齐齐哈尔大学青年科研启动支持计划(2010kM10)
齐齐哈尔市科技局项目(GYGG0811)
关键词
纤维素降解菌
协同效应
金属离子
cellulose-decomposing microorganisms
synergism
metal ions
作者简介
王志刚（1980-），男，内蒙古赤峰人，主要研究方向为环境微生物学。E—mai：wzgl980830@yahoo．com．cn；Tel：0452—2738704
通讯作者，徐伟慧，E—mail：xwh800206@163．com
