摘要
目的:维生素D(VD)对机体免疫功能作用已引起广泛关注,本实验研究补充不同剂量VD3对大鼠免疫细胞活性的影响。方法 :将30只Wistar大鼠随机分为3组,分别为VD31(对照组)、VD32和VD33组,每组10只,雌雄各半。VD31组大鼠每天摄入VD320 IU/kg(生理剂量,相当于成人每天摄入5μg维生素D3)、VD 2组和VD 3组分别补充200IU/(kg.d)(10倍量)和1 000IU/(kg.d)(50倍量)的VD3;补充时间为4周。实验结束时麻醉无痛处死,取血样(约5mL)离心,采用LC-MS/MS测定血清VD3浓度、CCK-8法检测淋巴细胞增殖活性、流式细胞术测单核细胞吞噬功能。结果:经过4周VD3补充后,结果显示,VD31、VD32和VD33组大鼠血清VD3浓度分别为14.8ng/mL、34.7ng/mL和60.7ng/mL,随着补充剂量的增加,机体VD3吸收水平也显著提高;VD3补充后,VD32组淋巴细胞增殖活性的OD值达到3.04,明显高于VD31组的2.64(P<0.05),但VD33组与VD31组相比淋巴细胞增殖活性无显著性差异(P>0.05)。采用流式细胞术观察单核细胞吞噬活性(荧光强度),结果显示VD32组大鼠单核细胞吞噬活性荧光强度到达19.98,明显高于VD31组的15.48,而高剂量VD3补充的VD33组大鼠单核细胞吞噬活性未见明显增强。结论 :适宜剂量VD3[200IU/(kg.d)]补充可增强淋巴细胞增殖和单核细胞吞噬活性,而过高剂量(50倍生理剂量)未观察到明显免疫调节作用。
[Objective] The effect of vitamin D （VD） on immune function has attracted wide attention. The objective of this experi- ment is to study the effect of different doses of vitamin D3 （ VD3 ） supplementation on immune cells activities in rats. [Method] Totally 30 Wistar rats were randomly divided into 3 groups： group VD3 1 （control group）, group VD3 2 and group VD3 3. Each group had 10 rats, male and female in equal. The daily intake of VD3 in group VD3 1 was 20 IU/ （ kg . d） （ equal to 5 μg/d vitamin D3 3 for adults）, the do- ses of VD3 supplemented to group VD3 2 and group VD3 3 were 200IU/ （ kg.d） and 1 000IU/ （ kg.d） respectively. The supplementation period was 4 weeks. At the end of the trial, rats were anesthetized to painless death, samples of whole blood （about 5mL） were collected and centrifuged. LC-MS/MS was used to detect serum vitamin D3. CCK - 8 method was used to detect lymphocyte proliferation activity and flow cytometry （FCM） was used to detect phagocytosis function of mononuclear cell. [Result] After 4 weeks supplementation of VD3, ser- um vitamin D concentration in VD3 1, VD3 2 and VD3 3 groups were 14. 8, 34. 7, and 60. 7 ng/mL respectively. Along with the increasing of supplementation doses, the amount of absorbed VD3 also increased remarkably. After supplementation of VD3, lymphocyte proliferation activity （OD） in group VD3 2 was 3.04 significantly higher than that of group VD3 1 which was 2.64 （ P 〈 0. 05 ）, while there were no sig- nificant differences between group VD3 3 and group VD3 1 （P 〉 0. 05 ） . Phagocytosis function of mononuclear cell （fluorescence intensity） were detected by FCM. The results showed that fluorescence intensity of group VD3 2 was 19. 98 significantly higher than that of group VD3 1 which was 15.48, while there were no significant increase of phagocytosis function of mononuclear cell in group VD3 3. [Conclusion] Appro- priate dose of VD3 [ 200IU/ （kg.d） ] supplementation might enhance lymphocyte proliferation activity and phagocytic function of mononu- clear cells, while exorbitant doses supplementation （50-fold physiological dose of VD3 ） failed to observe significant improvement in im- mune function.
出处
《中国食物与营养》
2012年第3期64-67,共4页
Food and Nutrition in China
基金
国家自然科学基金(项目编号:81172662)
作者简介
李敏（1985-），女，山东滨州人，在读硕士研究生，研究方向：应用营养。
马爱国（1956-），男，江苏宿迁人，博士，教授，研究方向：应用营养。
