摘要
目的研究升清胶囊定性定量方法。方法对大黄进行了显微鉴别采用TLC法对升清胶囊中虎杖及陈皮进行定性鉴别;采用HPLC法对方中大黄及虎杖的主要成分大黄素进行了定量分析。色谱柱为C18柱(250mm×4.6mm,5μm),流动相为甲醇-0.1%磷酸溶液(85∶15),流速1.0mL.min-1,检测波长为254nm,柱温为室温。结果定性鉴别明显清晰,阴性样品无干扰;大黄素对照品在0.048 5～0.388 0μg进样量与峰面积呈良好线性关系,平均加样回收率为99.2%,RSD=0.80%(n=6)。结论方法简便可行,结果准确可靠,重现性好,可作为本产品的质量控制方法。
Objective To establish the qualitative and quantitive methods for Shengqing Jiaonang.Method Rheum palmatum L.was identified by microscopic identification.Polygonum cuspidatum Sieb.et Zucc.and citrus reticulata Blanco were identified by TLC.The content of emodin was determined by HPLC.The separation was performed on C18 column with methanol-0.1% phosphoric acid solution（85∶15）as the mobile phase.The flow rate was 1.0 mL·min-1.The wavelength was 254 nm.The column temperature was room temperature.Results The TLC spots were fairly clear,and the negative sample showed no interference.The emodin was linear over 0.048 5-0.388 0 μg,the average recovery was 99.2%,and the RSD was 0.80%（n=6）.Conclusion The method is simple,reliable,accurate,and can be used as the quality control method for Shengqing Jiaonang.
出处
《中南药学》
CAS
2012年第3期192-195,共4页
Central South Pharmacy
作者简介
宋锦锋，女，主管药师，主要从事药品检验及分析工作，Tel：（0730）8875079，E-mail：191689027@qq．com
