摘要
采用简易CTAB法、改良CTAB法、SDS-CTAB法、高盐法和CTAB-硅珠法等5种方法对风信子花蕾、叶片和鳞片基因组DNA进行提取。比较DNA纯度、电泳、得率等指标,结果表明:CTAB-硅珠法没有获得DNA,其他方法均可提出DNA;在纯度方面,简易CTAB法>SDS-CTAB法>改良CTAB法>高盐法;在得率方面,简易CTAB法>改良CTAB法>高盐法>SDS-CTAB法。简易CTAB法提取的DNA纯度较高,OD260/OD280为2.01,OD260/OD230为2.33,浓度为406.8ng.μL-1,得率为175.95ng.μL-1。花蕾、叶片适合风信子基因组DNA的提取。
Five methods including simple CTAB,CTAB improved method,SDS-CTAB method,high salt precipitation method and silica-purification method were used to extract genomic DNA from different parts of Hyacinth such as bud,leaves,scale.The results were compared by three parts,purity,the electrophoreses results and yield of the extracted DNA.The results showed that all of these DNA extraction methods except for CTAB-silica-purification method could obtain DNA.The purity of extracted DNA was as following ： simple CTAB method SDS-CTAB method CTAB improved methodhigh salt precipitation method,and yield as following ： simple CTAB methodCTAB improved methodhigh salt precipitation methodSDS-CTAB method.Simple CTAB method could obtain more purity DNA than the other methods and OD260/OD280 was 2.01,OD260/OD230 was 2.33.The concentration was 406.8ng·L-1 and the yield was 175.95ng·g-1.The extraction results from different parts showed that flower buds and leaves were both suit for Hyacinth obtain DNA.
出处
《沈阳农业大学学报》
CAS
CSCD
北大核心
2011年第5期570-573,共4页
Journal of Shenyang Agricultural University
基金
国家林业局"948"项目(2009-4-12)
中国博士后科学基金面上项目(20100470217)
江苏省高校优势学科建设工程资助项目
作者简介
胡凤荣（1969-），女，南京林业大学副教授，北京林业大学博士后，从事园林植物遗传育种研究。
通讯作者：罗凤霞（1958-），女，金陵科技学院教授，硕士，从事园林植物遗传育种研究。
