摘要
目的 隐匿性乙型肝炎感染一直是困扰献血中健康血清筛查的一个重要因素,通过高度灵敏的Nest-PCR检测方法对献血者中的OBI进行调查,了解该地区隐匿性HBV感染情况和基因分析.方法 共收集10080例献血者血浆,进行进口雅培HBsAg试剂和北京万泰抗-HBc及抗-HBs试剂平行检测,采用高灵敏度的Nest-PCR进行核酸检测和基因调取.结果 10080例无偿献血者中,共检出雅培（超敏）HBsAg阳性108例（阳性率1.07％）,抗-HBC单独阳性767例（阳性率7.67％）.10080例献血者中筛查出25例血浆HBsAg检测阴性HBV DNA检测阳性的隐匿性HBV携带者.隐匿性HBV感染者的发生率为0.25％.HBV基因C型12例（48％）,基因B型13例（52％）,未发现其他基因型.基因B型和基因C型之间没有明显的统计区别（P＞0.05）,序列分析显示其中5例在HBsAg“a”表位（aa124～aa147）存在突变（20％）.结论 我国无偿献血中存在较高比例的隐匿性乙型肝炎感染,且不同地区的基因型与突变情况存在差异.
Objective Occult hepatitis B infection of voluntary blood donors has been plagued in the serum screening.Determined the OBI through the highly sensitive detection methods Nest-PCR among the blood donors,and then learned occult HBV infection and analysed the genotypes of this area.Methods 10 080 serums of donors were determined respectively by the imported Abbott HBsAg kit and Beijing Wantai anti-HBc and anti-HBs reagents,obtained the gene and detected DNA sequences by the high sensitive Nest-PCR method.Results Among 10 080 cases of unpaid blood donors,108 cases were detected HBsAg positively by Abbott sensitivity kit （ positive rate of 1.07% ）,767 cases were anti-HBc single - positive （ positive rate of 7.67% ）.25 patients screened blood donors who tested negative for serum HBsAg and positive for HBV DNA in the 10080 cases.Occult HBV infection incidence rate was 0.25%.12 cases were HBV genotype C （48%）,13 cases were genotype B （52%）,and no other genotypes.Genotype B has no statistically significant difference to genotype C （ P 〉 0.05 ）.Sequence analysis showed that 5 patients in the HBsAg epitope ＂a＂ （aa124 - aa147 ） have mutation （20%）.Conclusion The high proportion of occult hepatitis B infected among voluntary blood donors in our country.Also genotype and mutation was differences in different regions.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
北大核心
2011年第6期457-459,共3页
Chinese Journal of Experimental and Clinical Virology
关键词
肝炎病毒
乙型
感染
表位
突变
基因型
供血者
Hepatisis B vlrus
Infection
Epitopes
Mutation
Genotype
Blood donors
作者简介
通信作者：纪勇平，Email：jyp666888@yahoo．com．cn
