摘要
采用烟碱转化早期诱导方法,对白肋烟达所26及其自交后代不同烟株的烟碱转化率进行了分析测定,以确定烟碱转化株鉴别标准的可靠性和有效性。结果表明,烟碱转化率低于2.5%的烟株自交后代株系稳定,株间烟碱转化率变异小,且均低于2.5%,表明母代烟株为非转化株;烟碱转化率介于2.5%和3%的3个烟株自交后代均表现株间烟碱转化性状的分离,变异幅度分别为0.439%-10.331%、0.466%-21.834%和0.394%-3.757%,表明母代烟株为转化株。烟碱转化率大于3%的烟株自交后代烟碱转化率株间变异性大,烟碱转化性状分离严重;烟碱转化率大于50%的2个烟株自交后代烟碱转化率也都较高,是高转化株的表现。因此,烟碱转化率大于2.5%是确定转化株的可靠标准。
Early conversion stimulation method was used to analyze alkaloid composition and content in tobacco leaves of Dasuo 26 and their selfing populations in order to verify reliability and validity. Results showed that the selfing popula- tions of plants with percent nicotine conversion （PNC） lower than 2. 5 % was stable and all had PNC lower than 2.5 % , indicating that the parent plants were non-converter. The three selfing generations from parent plants with PNC between 2.5%and 3% showed significant segregation of the trait of nicotine conversion within the range of 0. 439%-10. 331% , 0. 466%-21. 834, and 0. 394%-3. 757%, respectively, which indicated that the parents were converters. The selfing populations from parent plants with PNC greater than 3 % showed great variation for nicotine conversion, and the 2 sel- fing lines from parents with PNC greater than 50% had the highest conversion level, indicating that the parents were high converters. Therefore, PNC greater than 2.5% can be a reliable standard to determine a converter plant.
出处
《中国烟草学报》
EI
CAS
CSCD
北大核心
2012年第1期29-34,共6页
Acta Tabacaria Sinica
基金
四川省烟草公司2007科技项目(SCYC2007-5-3)
关键词
白肋烟
烟碱
降烟碱
烟碱转化率
鉴别标准
burley
nicotine
nornicotine
percent nicotine conversion
identification standard
作者简介
赵晓丹（1985-），硕士研究生，主要从事烟草栽培和生理研究，E-mail：zxd397497717@163．com
通讯作者：史宏志（1963-），博士，教授，主要从事烟草栽培和生理研究，E-mail：shihongzhi88@163．com
