摘要
目的制备硒化枸杞多糖硫酸酯(Se-LBPS),考察其抗氧化活性和对人宫颈癌Hela细胞生长的抑制作用。方法以自制的枸杞多糖硫酸酯(LBPS)为原料,在乙酸催化下与亚硒酸钠反应,合成Se-LBPS。以付里叶红外光谱对产物进行表征。用分光光度法考察Se-LBPS的抗氧化活性,用MTT法考察其对人宫颈癌Hela细胞的体外抑制作用。结果 Se-LBPS对羟自由基的清除率可达60.51%,对超氧自由基的清除率可达47.9%。Se-LBPS对Hela细胞生长的抑制率优于LBPS和硒化枸杞多糖(Se-LBP),Se-LBPS对Hela细胞的抑制率与其浓度正相关,在200μg.mL-1时抑制率可达59.67%。结论 Se-LBPS具有明显的抗氧化性,优于硒化红薯叶多糖。Se-LBPS对Hela细胞生长具有显著的抑制作用,其抑制效果优于叶酸。
OBJECTIVE To prepare selened Lycium barbarum polysaccharides sulfates （Se-LBPS） and to investigate its antioxi- dation activity and inhibitory effect on Hela cell growth. METHODS Se-LBPS was prepared through reaction of laboratory-made sul- fated Lycium barbarum polysaccharides with sodium selenite using acedic acid as the catalyst, and it was characterized by means of Fourie transform infrared spectroscopy. The antioxidation activity was investigated by spectrophotometry, and the inhibitory action of Se- LBPS on the growth of human cervical carcinoma Hela cell in vitro was examined using MTF method. RESULTS The eliminating rati- os of hydroxyl radical and superoxide radical for Se-LBPS achieved 60. 51% and 47.9%, respectively. The growth inhibiton ratio of human cervical carcinoma Hela cell by Se-LBPS was better than LBPS and selened Lycium barbarum polysaccharides （Se-LBP）. The inhibition ratio was positively correlated with Se-LBPS concentration. An inhibition ratio of 59. 67% was achieved with 200 μg mL- Se-LBPS. CONCLUSION Se-LBPS has obvious antioxidation activity which is stonger than selenium sweet potatoleaf polysaccar- ides, and marked inhibitory effects on human cervical carcinoma Hela cell growth stonger than folic acid.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2012年第6期423-426,共4页
Chinese Pharmaceutical Journal
基金
河北省自然科学基金项目(B200500010)
关键词
枸杞多糖硫酸酯
硒化枸杞多糖硫酸酯
抗氧化活性
人宫颈癌HELA细胞
抑制作用
lycium barbarum polysaccharides sulfates （LBPS） selened lycium barbarum polysaccharide sulfates （Se-LBPS） an-tioxidation activity human cervical carcinoma hela cell inhibitory effect
作者简介
赵燕燕，女，教授，硕士生导师研究方向：药动学和药效学
孙汉文，男，教授，博士生导师研究方向：药物分析
