摘要
目的:研究大黄提取后浓缩干燥过程中各活性成分的稳定性。方法:以大黄提取液中各活性成分为研究对象,在浓缩干燥过程中不同时间点取样,HPLC分别对其测定,检测相对含量变化趋势。结果:在70℃受热过程中,各活性成分相对含量在浓缩1 h后降解程度较大,浓缩2 h与1 h后相比减少不明显,浓缩3 h后含量则显著降低;70℃减压干燥过程中,各活性成分含量在干燥2 h后降解程度较大,干燥3 h与干燥2 h相比变化不大,总趋势相对较稳定,干燥过程对各活性成分含量影响较小。番泻苷D受热易分解。结论:大黄提取液在70℃减压浓缩干燥过程中,浓缩时间宜控制在2 h之内,各成分在干燥3 h过程内稳定。
Objective： To study on stability of active ingredients from Rheum officinale in concentration and drying process. Method： Taking active ingredients from extraction fluid of R. officinale as studying target, samples were taken at different time points in concentration and drying progress, change trends of relative content was determined by HPLC. Result： The relative content of active ingredients were degraded greater degree after concentrated 1 h in process of heating at 70 ~C, its reduction is not obvious after concentrated 2 h by comparing with 1 h, but after concentrated for 3 h, the relative content reduced significantly; At 70 ~C vacuum drying process, active ingredients were in large degree of degradation after drying 2 h, the change of content was small after drying 3 h by comparing with 2 h, general trend was relative stable, the content of active ingredients was less affected in drying process. Sennoside D decomposed easily in heating. Conclusion： During in process of concentration and drying at 70 ~C vacuum for extraction fluid from R. officinale , concentration time should be controlled within 2 h, components were stable in drying time for 3 h.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第6期10-12,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
"十一五"国家重大新药创制课题(2010ZX09401-406)
关键词
大黄
浓缩干燥
活性成分
峰归属
稳定性
Rheum officinale
concentration and drying
active ingredients
peaks ascription
stability
作者简介
哈飞，硕士研究生，从事药物分析研究，Tel：022-86342546，E-mail：hafeikaoyan@126．com
[通讯作者]张兰兰，博士，从事新药开发研究，Tel：022—86342608，E-mail：zhang112@tasly．com
