摘要
【目的】克隆桑树肌动蛋白MaACT3的启动子。【方法】采用抑制PCR技术获得肌动蛋白的5′端侧翼序列;采用农杆菌感染成熟叶片瞬时表达检测启动子活性。【结果】获得的5′端侧翼序列含有基因的翻译起始位点、第一外显子、第一内含子和第二外显子的一部分,外显子部分与已报道的桑树肌动蛋白MaACT3序列完全相同。通过荧光显微镜观察到了绿色荧光,在mRNA水平上也检测到表达,证实了获得的5′端序列具有启动活性。【结论】获得的桑树肌动蛋白MaACT3的启动子具有启动活性,可以应用于桑树转基因研究。
【Objective】 The objective of this study is to clone the promoter of MaACT3 from mulberry（Morus alba）.【Method】The promoter of MaACT3 was obtained by suppression PCR method and the activity of promoter was valued by agrobacterium-mediated transient expression.【Result】 The sequence analysis indicated that exon I,intron I and partial exon II were included and the partial exon II was the same as mRNA of MaACT3 reported before.Mature leaves were used for agrobacterium-mediated transient expression.Green fluorescence was measured by fluorescence microscope and mRNA was also detected.【Conclusion】 The promoter of MaACT3 from mulberry expressed promoter activity and could be used in transgenic research of mulberry.
出处
《中国农业科学》
CAS
CSCD
北大核心
2012年第4期625-632,共8页
Scientia Agricultura Sinica
基金
国家现代农业产业技术体系建设专项(nycytx-27-gw101)
国家自然青年基金(31101769)
重庆市蚕桑重大科技专项(CSTC
2009AA1024)
作者简介
李军，E-mail：speker@163．com。
赵爱春为同等贡献作者，Tel：023．68250793；E-mail：zhaoaichun@hotmail．com。
通信作者余茂德，Tel：023-68250191；E-mail：yumd@163．com
