摘要
目的观察达肝清颗粒对大鼠的长期毒性。方法大鼠80只,分为达肝清颗粒高、中、低剂量组(以生药计分别为170.08,5.0和42.5 g.kg-1)及空白对照组,每组20只,每日灌胃给药1次,空白对照组给予等体积纯化水,连续给药90 d。观察大鼠一般状况和给药后90 d及恢复期14 d结束后血液学、血液生化学、脏器系数及组织病理学变化。结果达肝清颗粒170.0 g.kg-1组大鼠体质量增长略慢,但与空白对照组比较差异无统计学意义,组织病理学检查发现有肝脏灶性中度脂肪变性8只和弥漫性中度脂肪变性1只,停药14 d后可恢复;连续给药90 d后达肝清颗粒各剂量组血清肌酐含量明显降低(P<0.05),停药后均可恢复。其余各剂量组大鼠体质量、摄食量、生化指标、脏器系数和病理学检查与空白对照组比较,均差异无统计学意义。结论达肝清颗粒按拟定的临床剂量、给药途径和疗程应用比较安全。
Objective To investigate long-term toxicity of daganqing granules in rats.Methods The total of 60 Wistar rats were randomly divided into the high,middle and low dose groups,which were treated with daganqing granules（170.0,85.0 and 42.5 g ·kg-1·d-1 crude drug） by intragastric administration,respectively,once a day for 90 days.The general behaviors and physical status,hematology,blood biochemistry,organs coefficient and histopathological analysis were detected in 90 days and 14 days after drug withdrawn.Results No significant difference in body weight was found between treated rats and the blank control despite the rats treated with 170.0 g·kg-1.There were focal moderate fatty infiltration developed in eight rats and diffuse moderate fatty infiltration in one rat by histopathological examination.However,these symptoms subsided in 14 days after medication termination.The serum creatinine was significantly reduced after consecutive 90 days＇therapy of daganqing granules compared with that in the control group（P0.05）,while which were reversed to normal in 14 days after supplements termination.There were no significant differences observed between two other treating groups and the control in the general behaviors and physical status,the hematology,blood biochemistry,organs coefficient and histopathological status.Conclusion Daganqing granules are safe by intragastric administration at the dosage in clinical range.
出处
《医药导报》
CAS
北大核心
2012年第3期286-288,共3页
Herald of Medicine
基金
广西壮族自治区科技厅基金资助项目(07109004-5Z)
关键词
达肝清颗粒
毒性
长期
脂肪变性
肌酐
Daganqing granules Toxicity long-term Fatty infiltration Creatinine
作者简介
曾春晖（1974-），女，广西钦州人，副教授，硕士生导师，硕士，从事中草药炮制与药理研究。电话：0771—2219854，E—mail：ehhzeng@163．com。
通讯作者杨柯（1975-），男，侗族，广西柳州人，副教授，硕士生导师，硕士，从事中草药药理学研究。电话：0771-2279423．E-mail：kyang_l1@126．com。
