摘要
利用ISSR技术分析了广东豚草(Ambrosia artemisiifolia L.)居群的遗传多样性及遗传分化。应用11条引物从6个居群182个样品中扩增出186个位点,多态性位点比率(PPB%)为98.92%。Shannon信息指数(I)为0.5238,Nei’s基因多样性指数(H)为0.3540,反映了广东豚草居群具有较高的遗传多样性。基因分化系数(GST=0.3263)和AMOVA分析均显示入侵广东的豚草居群具有很高的遗传分化。研究结果表明,粤北与粤东豚草居群的遗传距离较近,最南部的花都居群(HD)与其他居群遗传距离相对较远,说明向南部扩散的豚草居群与粤北的居群已产生了较大的遗传分化。
The population genetic diversity and differentiation of the invasive species,Ambrosia artemisiifolia,in Guangdong were analyzed using ISSR.A total of 11 primers were used and 186 bands were amplified from 182 samples of the six populations.The percentage of polymorphic loci was 98.92%.Shannon's information index was 0.5288.Nei's index was 0.3540.The data suggested genetic diversity of Guangdong populations was high.Both GST(0.3263) and AMOVA showed a high differentiation among populations.The populations in the northern and eastern parts of Guangdong had close genetic similarity,but the southernmost population of Huadu had higher genetic differentiation from other populations.It is indicated that the southern population has developed higher genetic divergence with its expanding southward.
出处
《广东农业科学》
CAS
CSCD
北大核心
2012年第3期135-138,共4页
Guangdong Agricultural Sciences
基金
广东省植物防疫检疫科研专项基金(4400-F09044)
科技部基础专项(4200-A07054)
关键词
豚草
入侵植物
遗传多样性
ISSR
Ambrosia artemisiifolia L.
invasive plant
genetic diversity
ISSR
作者简介
黄久香（1975-），女，博士，副教授，E—mail：jxhuang@seau．edu．cn
通讯作者：庄雪影（1961-），女，博士，教授，E-mail：xyzhuang@$cau．edu．cn
