摘要
目的探讨黑龙江省乙型肝炎病毒（HBV）拉米夫定耐药株YMDD变异与基因型、HBVBCP变异之间的相关性。方法对收集的245例经拉米夫定治疗的慢性乙型肝炎患者血清标本，采用多重PCR法检测HBV的基因型；荧光标记杂交双探针PCR融解曲线法（FH—PCR—MC）检测HBVYMDD变异及变异类型；巢式PCR法扩增HBVC基因区，测序分析BCP位点变异并进行统计学分析。结果在HBVYMDD野生株、变异株中，基因型B、C、B与C混合型分别为8．8％、89．2％、2．0％；6．3％、93％、0．7％，基因型构成比无统计学的差异。HBVBCP的变异率在YMDD野生株与变异株分别为69．6％、76．9％，无统计学的差异。在HBVYMDD野生株中，HBVB基因型BCP的变异率22．2％，C基因型BCP的变异率73．6％存在统计学差异（P〈0．01），但在YMDD变异株中，无统计学的差异。在YIDD、YVDD变异株HBVBCP变异率分别为83．0％和64．6％，存在统计学的差异（P〈0．05）。结论在HBVYMDD野生株中，与B基因型相比，C基因型更易发生BCP变异；YMDD变异株中，BCP变异率在B、C基因型间无统计学差异，BCP变异在变异类型间（YIDD、YVDD）存在统计学差异；与YVDD变异株相比较，YIDD变异株易发生HBVBCP的变异。
Objective To investigate the correlation between BCP mutations ,.genotypes and HBV Lamivudine-resistant YMDD mutation in Heilongjiang province. Methods The HBV DNA in 245 sera samples were collected from chronic hepatitis B patients who had been treated with lamivudine. HBV geno- types and YMDD mutations were detected by multiplex PCR and FH-PCR-MC respectively. HBV C gene re- gion was amplified by nest PCR, then BCP mutations were analyzed. Results There were no statistical differences for the ratio of genotype between YMDD wild type（ 8. 8% with genotype B ,89. 2% with genotype C and 2. 0% with B ,C mixed genotype）and YMDD mutant type（6. 3% with genotype B ,93.0% with genotype C and O. 7% with B,C mixed genotype）. The ratio of BCP mutations in YMDD mutant type and YMDD wild type were 76. 9% and 69. 6% respectively and there were no statistical differences between them. In YMDD wild strains, BCP mutations occurred more frequently in genotype C than in genotype B （P 〈 0. 01 ）.While, in YMDD mutant type, there was no significant difference. The ratio of BCP mutations were 83.0% and 64. 6% between YIDD and YVDD mutants, respectively, there were statistical differences between them （ P 〈 0. 05 ）. Conclusion There was a significant difference of the HBV BCP mutation with YMDD wild strains between the genotypes B and C . Mutation rate of the HBV BCP with YMDD mutants showed no significant difference between genotypes B and C . There was a significant difference of the HBV BCP mutation with YMDD mutants between the YIDD mutation type and YVDD mutation type.
出处
《国际病毒学杂志》
2012年第1期15-19,共5页
International Journal of Virology
基金
哈尔滨市科技创新人才研究专项资金项目青年科技创新人才（2008RFQXS084）
作者简介
通讯作者：李迪，E—mail：chlidi@hotmail．com
