摘要
目的探讨复方血栓通胶囊对糖尿病大鼠视网膜组织抗缺氧及抗氧化能力的干预作用．并研究其作用机制。方法实验研究。雄性SPF级SD大鼠110只，适应性饲养7d后随机取30只作为正常组。余80只以链脲佐菌素（STZ）诱导制作糖尿病大鼠模型，造模成功66只，取其中60只,随机分成两组：30只大鼠不予处理，作为对照组；其余30只作为治疗组，给予复方血栓通胶囊按900mg／kg／d的剂量进行治疗。在第4、第12、第20周到达实验终点时，采用免疫组织化学、分光光度法和1α-PCR方法分别检测各组视网膜组织中缺氧诱导因子-1α（HIF-1α）和铜锌超氧化物歧化酶（CuZn-SOD）的阳性细胞数及mRNA水平表达量，以及视网膜组织中超氧化物歧化酶（SOD）的活力。对相关数据行随机区组设计的方差分析。结果①实验第4、第12和第20周，对照组糖尿病大鼠视网膜节细胞层及内核层均可见大量HIF-α阳性细胞表达，而治疗组HIF-1α阳性细胞数较同时间点的对照组少（P〈0．051：在各时间点，治疗组HIF-10αmRNA的表达也较对照组低，差异均有统计学意义（P〈O．05）。②第4、第12和第20周时，治疗组CuZn-SOD阳性细胞数较对照组多，CuZn．SODmRNA的表达也较对照组高，差异均有统计学意义（P〈0．05）。③第4周和第12周时，治疗组的总超氧化物歧化酶（T-SOD）和CuZn-SOD活力较对照组有显著提高，差异均有统计学意义（P〈0．05）。结论复方血栓通胶囊可能是通过改善组织缺血缺氧状态，降低HIF-1α表达，提高SOD活力和CuZn-SOD蛋白表达水平，从而来提高早期糖尿病大鼠视网膜组织抗缺氧及抗氧化能力。
Objective To investigate the anti-hypoxia and anti-oxidation effect of Fufang Xueshuantong capsule on the retina of rats with diabetic retinopathy and explore its mechanism. Methods It was an experimental study. One hundred and ten male SPF Sprague Dawley rats were raised for 7 days and 30 of them were randomized as the normal group. And the other 80 rats were induced by Streptozotocin, randomized 60 successfully induced diabetic rats, which were divided into two groups： 30 STZ-diabetie rats without treatment as the diabetic rat control group; 30 STZ-diabetic rats treated with Fufang Xueshuantong capsule at a dose of 900 mg/kg/day as the treatment group. After the animals were raised for 4 weeks, 12 weeks, 20 weeks, use immunohistochemistry method and RT-PCR to detect the expression levels of hypoxia inducible factor-1α （HIF-1α and CuZn super oxide dismutase （CuZn-SOD） proteins and mRNAs in the retina; determining the activities of T-SOD and CuZn proteins in the retina of rats in each group by a spectrophotometric method seperately. Data were analyzed using randomized block analysis of variance. Results ①At 4, 12 and 20 weeks after treatment, the expression of HIF-1α protein were founded in the purkinje layer and innernuclear layer of diabetic rats, and decreased in the treatment group （P〈0.05）. The expression level of HIF-1α mRNAs was also lower in the retina of diabetic rats than in the treatment group （P〈0.05）. ②At 4, 12 weeks and 20 weeks after treatment, the expression of CuZn-SOD protein in the purkinje layer and inner nuclear layer of diabetic rats was obviously lower than the normal group＇s, and was higer than the treatment group＇s （P〈0.05）. The expression of CuZn-SOD mRNA was also higer in treatment group than in control group （P〈0.05）. ③At 4 weeks and 12 weeks, the activities of T-SOD and CuZn-SOD were decreased in diabetic rats compared with normal groups, but obviously increased in the treatment group （P〈0.05）. Conclusion The anti-oxidation and anti-hypoxia effect of Fufang Xueshuantong capsule on the retina of rats with early diabetes maybe mediated by ameliorating the state of ischemia and hypoxic, downregulating the expression of HIF-1α, enhancing the activity of SOD and upregulating the expression of CuZn-SOD.
出处
《中华眼视光学与视觉科学杂志》
CAS
2012年第1期41-46,共6页
Chinese Journal Of Optometry Ophthalmology And Visual Science
基金
广东省东莞市科技计划基金资助项目（2004D1036）
关键词
复方血栓通胶囊
中草药
糖尿病视网膜病变
缺氧诱导因子1
仅亚基
超氧化
物歧化酶
模型
动物
免疫组织化学
Fufang Xueshuantong capsule
Drugs,Chinese Herbal
Diabetic retinopathy
Hypoxia-inducible factor 1,alpha subunit
Superoxide dismutase
Models,animal
Immunohistochemistry
作者简介
通信作者：于强，Email：yuqiang-gd@tom．com
