期刊文献+

南蝠线粒体DNA控制区结构及贵州7个种群遗传多样性的分析研究

Structure of the Mitochondrial DNA Control Region and Genetic Diversity Analysis of Seven Ia io Populations in Guizhou
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摘要 采用PCR技术获得了贵州7个南蝠(Ia io)自然种群42个个体的线粒体DNA控制区全序列,长度为1256~1340 bp.对控制区结构进行分析,识别了其延伸的终止结合序列区(包括ETAS1和ETAS2元件)、中央保守区(包括F、E、D、C、B元件)和保守序列区(包括CSB1、CSB2和CSB3元件);同时,在延伸的终止结合序列区还发现了若干能形成发夹结构的主体序列TACAT—ATGTA.在7个自然种群42个个体中共定义了16个单倍型.遗传多样性分析表明:贵州南蝠种群具有较高的单倍型多样性(h=0.945)和中等的核苷酸多样性(π=0.012).基因流、AMOVA和系统进化树分析表明贵州这7个南蝠自然群体间没有发生遗传分化. The sequences of the control region (D-loop) of mitochondrial DNA (mtDNA) were obtained from 42 individuals of seven I.io populations from Guizhou province. The lengths of D-loop region are 1 256-1 340 bp. It could be divided into three domains, the extended termination associated sequence (including ETASland ETAS2), the central conserved sequence domain (including F, E, D, C, B boxes) and the conserved sequence block domain (including CSB1, CSB2, CSB3). And a sequence (TACAT) and its reverse complementary se- quence (ATGTA) were found in the ETAS domain. 16 haplotypes were identified from 42 individuals of 7 I.io populations. I.io populations from Guizhou have high haplotype diversity (h=0.945) and medium nu- cleotide diversity (П=0.012). Gene flow, AMOVA and phylogenetic tree show that there is no genetic differ- entiation among those populations.
出处 《生命科学研究》 CAS CSCD 北大核心 2012年第1期33-41,共9页 Life Science Research
基金 贵州省优秀科技教育人才省长专项基金资助项目(黔省长合字(2009)117)
关键词 南蝠 MTDNA 控制区结构 遗传多样性 贵州 Ia io mtDNA structure of D-loop genetic diversity Guizhou (Life Science Research, 2012, 16(1) : 033~041)
作者简介 田东(1986-),男,贵州德江人,硕士研究生,主要从事动物遗传与进化研究,E-mail:txd768@yahoo.com.cn. 谷晓明(1954-),男,贵州贵阳人,贵州师范大学教授,主要从事分子进化与分子系统研究,E-mail:gxmswx@263.net.
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参考文献24

  • 1CHEN S F, ROSSITER S J, FAULKES C G, et al. Population genetic structure and demographic history of the endemic For- mosan lesser horseshoe bat (Rhinolophus monoceros)[J].Molec- ular Ecology, 2006, 15 (6) : 1643-1656.
  • 2RUSSELL A L, MEDELLIN R A, MCCRACKEN G F. Genetic variation and migration in the Mexican free-tailed bat(Tadarida brasiliensis mexicarta)[J].Molecular Ecology, 2005, 14 (7): 2207-2222.
  • 3XU Li-jie, HE Chun-feng, SHEN Cen, et al. Phylogeography and population genetic structure of the great leaf-nosed bat (Hipposideros armiger) in China[J]. Journal of Heredity, 2010, 101(5): 562-572.
  • 4SALGUEIRO P, COELHO M M, PALMEIRIM J M, et al. Mi- tochondrial DNA variation and population structure of the is- land endemic Azorean bat (Nyctalus azoreum)[J]. Molecular E- cology, 2004, 13(11): 3357-3366.
  • 5WALBERG M W, CLAYTON D A. Sequence and properties of the human KB cell and mouse L cell D-loop regions of mito- chondrial DNA[J]. Nucleic Acids Research, 1981, 9(20): 5411- 5430.
  • 6SBISAE, TANZARIELLO F, REYES A, et al.Mammalian mi- tochondrial D-loop region structural analysis: identification of new conserved sequences and their functional and evolutionary implications[J].Gene, 1997, 205 (1-2): 125-140.
  • 7SUN Ke-ping, JIANG Feng, JIN Long-ru, et al. Structure, DNA sequence variation and phylogenetic implications of the mito- chondrial control region in horseshoe bats[J] .Mammalian Biolo- gy, 2009, 74: 130-144.
  • 8刘丰,宋雨,颜识涵,罗金红,冯江.大趾鼠耳蝠线粒体DNA控制区结构及变异[J].动物学杂志,2009,44(4):19-27. 被引量:2
  • 9PETRI B, VON H A, PAEAEBO S. Extreme sequence hetero- plasmy in bat mitochondrial DNA[J]. Biological Chemistry Hoppe Sefier, 1996, 377: 661-667.
  • 10SOUTHERN O, SOUTHERN P J, DIZON A E .Molecular characterization of a cloned dolphin mitochondrial genome[J]. Journal of Molecular Evolution, 1988, 28(1): 32-42.

二级参考文献49

  • 1王俊森,柳劲松,张虹,杨光,徐德,马兰香,王主章,吴文谦.水鼠耳蝠的染色体组型研究[J].野生动物,1996,17(1):26-28. 被引量:7
  • 2张维道.绒山蝠的核型和C带初步研究[J].安徽师大学报,1990,13(4):58-63. 被引量:11
  • 3张维道.灰伏翼的染色体分析[J].遗传,1986,8(5):44-45.
  • 4Field D,Wills C.Long,polymorphic microsatellites in simple organisms.Proc Biol Sci,1996,263(1367):209-215.
  • 5Hancock JM.Simple sequences in a"minimal"genome.Nat Genet,1996,14(1):14-15.
  • 6Tóth G,Gáspári Z,Jurka J.Microsatellites in different eukaryotic genomes:survey and analysis.Genome Res,2000,10(7):967-981.
  • 7Gur-Arie R,Cohen CJ,Eitan Y,Shelef L,Hallerman EM,Kashi Y.Simple sequence repeats in Escherichia coli:abundance,distribution,composition,and polymorphism.Genome Res,2000,10(1):62-71.
  • 8International Human Genome Sequencing Consortium.Initial sequencing and analysis of the human genome.Nature,2001,409(6822):860-921.
  • 9Subramanian S,Mishra RK,Singh L.Genome-wide analysis of microsatellite repeats in humans:their abundance and density in specific genomic regions.Genome Biol,2003,4(2):R13.
  • 10Warren ST,Nelson DL.Trinucleotide repeat expansions in neurological disease.Curr Opin Neurobiol,1993,3(5):752-759.

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