摘要
采用PCR技术获得了贵州7个南蝠(Ia io)自然种群42个个体的线粒体DNA控制区全序列,长度为1256~1340 bp.对控制区结构进行分析,识别了其延伸的终止结合序列区(包括ETAS1和ETAS2元件)、中央保守区(包括F、E、D、C、B元件)和保守序列区(包括CSB1、CSB2和CSB3元件);同时,在延伸的终止结合序列区还发现了若干能形成发夹结构的主体序列TACAT—ATGTA.在7个自然种群42个个体中共定义了16个单倍型.遗传多样性分析表明:贵州南蝠种群具有较高的单倍型多样性(h=0.945)和中等的核苷酸多样性(π=0.012).基因流、AMOVA和系统进化树分析表明贵州这7个南蝠自然群体间没有发生遗传分化.
The sequences of the control region (D-loop) of mitochondrial DNA (mtDNA) were obtained from 42 individuals of seven I.io populations from Guizhou province. The lengths of D-loop region are 1 256-1 340 bp. It could be divided into three domains, the extended termination associated sequence (including ETASland ETAS2), the central conserved sequence domain (including F, E, D, C, B boxes) and the conserved sequence block domain (including CSB1, CSB2, CSB3). And a sequence (TACAT) and its reverse complementary se- quence (ATGTA) were found in the ETAS domain. 16 haplotypes were identified from 42 individuals of 7 I.io populations. I.io populations from Guizhou have high haplotype diversity (h=0.945) and medium nu- cleotide diversity (П=0.012). Gene flow, AMOVA and phylogenetic tree show that there is no genetic differ- entiation among those populations.
出处
《生命科学研究》
CAS
CSCD
北大核心
2012年第1期33-41,共9页
Life Science Research
基金
贵州省优秀科技教育人才省长专项基金资助项目(黔省长合字(2009)117)
关键词
南蝠
MTDNA
控制区结构
遗传多样性
贵州
Ia io
mtDNA
structure of D-loop
genetic diversity
Guizhou (Life Science Research, 2012, 16(1) : 033~041)
作者简介
田东(1986-),男,贵州德江人,硕士研究生,主要从事动物遗传与进化研究,E-mail:txd768@yahoo.com.cn.
谷晓明(1954-),男,贵州贵阳人,贵州师范大学教授,主要从事分子进化与分子系统研究,E-mail:gxmswx@263.net.