摘要
应用PCR方法对致病性外瓶霉进行分类鉴定。以ITS3和ITS4为引物 ,对常见的7种致病性外瓶霉的模式株核糖体DNA转录间隔区(ITSⅡ)进行扩增 ,4种内切酶(HinFI、MspI、BsuRI和RsaI)酶切。各种间多态性显著 ,常规方法难以鉴别的皮炎外瓶霉和甄氏外瓶霉较易区分。PCR RFLP准确可靠 ,可用于形态及其他方法难以确定的致病菌种的鉴别。
To analyze and identify the human pathogenic Exophiala spp. by using PCR RFLP. The ribosomal internal transcribed spacer regions(ITSⅡ)in 7 species were amplified by primer ITS 3 and ITS 4 and digested with four restriction enzymes(HinF I,Msp I,BsuR I and Rsa I). Characteristic patterns for the species were found after restriction analysis. PCR RFLP could be used to the identification of Exophiala spp. especially E. dermatitidis and E. jeanselmei.
出处
《临床皮肤科杂志》
CAS
CSCD
北大核心
2000年第1期10-12,共3页
Journal of Clinical Dermatology
基金
教育部博士学科点专项基金
