摘要
采用石蜡切片,HE组织染色方法研究性成熟刀鲚的精巢结构、精巢发育规律和精子形成过程。结果表明:刀鲚的雄性生殖腺由精巢(生精部)、贮精囊和输精管等组成,精巢为典型的小叶型结构,由精小叶、精小囊、小叶间质、小叶腔和输出管构成。精小叶由各期生精细胞和支持细胞构成,各期生精细胞和支持细胞组成了精小囊,成熟的精子从精小囊中释放出来,进入小叶腔中,经由精小叶间形成的临时通道进入输出管,再由输出管送达贮精囊。贮精囊为网状管腔结构,管腔内壁由分泌细胞组成。精子发生经历了精原细胞、初级精母细胞、次级精母细胞及精子细胞和成熟的精子5个阶段,根据精巢的外形、色泽、体积、血管分布状况等特征分为6个时期。
The testis structure,development regularity and spermiogenesis of sexually mature Coilia nasus were studied by using paraffin sections and H. E. staining method. The results indicated that, the male gonad of Coilia nasus was composed of testis （sperm production）, seminal vesicle and spermatic ducl. The spermary had typical lobular structures, which is composed of seminiferous lobula, follicle, interstitial substance, lobular cavity and efferent duct. The seminiferous lobula was composed of spermatogenic cells of varied stages and supporting cells,and spermatogenic cells of varied stages and supporting cells formed the follicle. The mature sperm was released from the follicle into the lobular cavity, then en- tered into efferent duct via transient pathway formed between lobes,and then was sent into the seminal receptacle. The seminal receptacle was in reticulated structure, and the inner wall was composed of excre ring cells. The sperms develop through 5 stages, including androgone, first spermatocyte, secondary spermatocyte, spermatoblast and mature sperms. Six stages were classified according to the appearance, color,volume and vascular distribution of the spermary.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2012年第2期247-252,共6页
Journal of Huazhong Agricultural University
基金
国家科技支撑计划项目(2012BAD26B00)
公益性行业(农业)科研专项(201203065)
江苏省科技支撑计划(农业)项目(BE2011411)
中国水产科学研究院淡水生态与健康养殖重点开放实验室开放课题(2010FEA03008)
关键词
刀鲚
精巢发育
形态
组织学
Coila nasus
development of testis
morphology
histology
作者简介
徐钢春，助理研究员．研究方向：鱼类繁殖生理学及养殖技术．E-mail：xugc@fire．cn
通讯作者：顾若波，研究员．研究方向：水产生物繁育及养殖技术．E-mail：gurb@fire．cn
