摘要
目的:了解1组(60株)耐药大肠埃希菌的菌株亲缘性。方法:对该组耐药大肠埃希菌4种与耐药相关的管家基因和45种水平转移获得与β-内酰胺类、氨基糖苷类、喹诺酮类耐药相关基因以及7种整合子、转座子、插入序列、接合性质粒遗传标记进行检测。并对检测结果进行样本聚类分析。结果:60株耐药大肠埃希菌多态性明显,多数菌株已发生演化。只有6、53号株;10、14号株,7、8、26、40号株3组携带相同基因(同一克隆)。结论:耐药基因的样本聚类分析可识别出具有相同耐药特征的菌株,具有一定的流行病学意义,为追溯耐药菌传播途径提供方便。
Objective： To investigate the relatedness in 60 drug-resistant Escherichia coli（E.coli） strains.Methods： Drug-resistance related genes were analyzed by PCR,including 4 kinds of housekeeping genes and 45 kinds of horizontal transfered genes related to 3 kinds of antimicrobial agents：beta-lactams,aminoglycosides,quinolones,as well as 7 kinds of genetic markers of integrons,transposons,insertion sequences and conjugal plasmids.They were used for the sample clustering analysis.Results： Polymorphism were distinct in 60 E.coli strains.Most strains showed evolution.Only 3 strain groups which consisted of strain 6 and 53,strain 10 and 14,strain 7,8,26 and 40,were the same clones carried the same genes.Conclusion： Sample clustering analysis of drug-resistance related genes could distinguish the strains with the same antimicrobial resistance characteristics,which was significant in epidemiologic research and provide convenience for tracing transmissible routes of drug-resistant strains.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2012年第1期109-114,共6页
Journal of Nanjing Medical University(Natural Sciences)
基金
江苏省实验诊断学重点实验室重大项目(XK200731)
作者简介
通讯作者:潘世扬。E-mail:sypan@njmu.edu.cn
