摘要
[目的]研究不同月份厚朴叶多糖体外对羟自由基(.OH)的清除作用。[方法]以不同月份厚朴叶为原料,通过提取及分离纯化得到厚朴叶多糖,采用分光光度法测定不同月份厚朴叶多糖对Fenton反应产生的羟自由基(.OH)的清除能力,并与抗坏血酸(VC)的抗氧化性进行了比较。[结果]不同月份厚朴叶多糖对羟自由基的清除率随多糖浓度的增加而增强;与抗坏血酸(VC)比较,厚朴叶多糖具有更强的清除羟自由基能力,8月份厚朴叶多糖的半抑制率IC50为0.08 g/L,相当于0.12 g/LVC对羟自由基的清除率;同等浓度条件下,10月份厚朴叶多糖在较高浓度时比抗坏血酸(VC)的抗氧化活性更高。
[ Objective ] The research aimed to analyze the effect of the polysaccharide from the magnolia leaves in different months on scavenging the hydroxyl radical （ ·OH） in vivo. [ Methods] With the magnolia leaves in different months as the tested materials, the polysaccharide from the magnolia leaves was obtained through the extraction, separation and purification. The ability of the polysaccharide from different months to remove the hydroxyl radical （· OH） produced by Fenion reaction was determined by the spectrometry and was compared with the oxidation resistance of Vc. [ Results] The scavenging rate of the polysaccharide from the magnolia leaves in different months on the hydroxyl radical was enhanced with the increment of the polysaccharide concn. Compared with the Vc, the polysaccharide from the magnolia leaves had the greater ability to remove the hydroxyl radical. The half inhibition rate IC50 of the polysaccharide from the magnolia leaves in August was 0.08 g/L, being equivalent to the scavenging rate of 0.12 g/L Vc agaisnt the hydroxyl radicals. Under the condition of the same concn., the polysaccharide at higher concn, from the magnolia leaves in October was higher than the Vc on the antioxidation activity. [ Conclusion ] The polysaccharide from the magnolia leaves in different months had Obvious inhibition on the hydroxyl radicals in the tested concn, range.
出处
《安徽农业科学》
CAS
北大核心
2011年第36期22303-22304,共2页
Journal of Anhui Agricultural Sciences
基金
陕西理工学院2011年大学生创新实验项目(UIRP1131)
关键词
厚朴叶
多糖
羟自由基
分光光度法
Magnolia leaves
Polysaccharide
Hydroxyl radical
Spectrophotometry
作者简介
邓芬（1987-），女，陕西白河人，专业：应用化学，E-mail：fangfang9933@126．com。
通讯作者，副教授，硕士生导师，从事绿色功能食品化学研究，E-mail：liucf@snut．edu.cn。
