摘要
采用树脂吸附原位分离技术生物发酵合成天然2-苯乙醇。利用气相色谱-质谱联用技术对纯化后的产物进行分析,结果表明,纯化后的2-苯乙醇产品纯度为99.49%。阈值分析结果表明,纯化后的2-苯乙醇香气强度适中,与从玫瑰精油中提取的天然2-苯乙醇的识别阈值(RT)相差0.33μg/L。电子鼻中的PCA分析能将不同来源的2-苯乙醇明显地区分开来。纯化后的2-苯乙醇与天然提取2-苯乙醇香气差异最小,相似系数为95.88%。
2-phenylethanol（2-PE） was fermented with an in situ product adsorption technique（ISPR） by Sac-charomyces cerevisiae AS 2.1182.After being isolated and purified,the product was detected by gas chromatography-mass spectrometry（GC-MS）,and the purity of 2-PE achieved 99.49%.The odor detection threshold of 2-PE synthesized by different ways was tested.Results indicated that recognition threshold（RT） of 2-PE produced by fermentation was moderate and differed 0.33 ag/L from that of 2-PE extracted from rose essential oil.Additionally,odoriferous quality of 2-PE acquired from different sources was identified by electronic nose.The odoriferous quality of 2-PE derived from fermentation was close enough to that extracted from rose with a similarity of 95.88%.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2011年第11期198-202,共5页
Food and Fermentation Industries
基金
上海市教委科研创新项目(09YZ394)
上海市科委国际合作项目(09540704200)
作者简介
硕士研究生
（荣绍丰副教授为通讯作者）。
