摘要
This study compares the changes of DNA repair in brain tissue dominated by the middle cerebral artery after electroacupuncture at the acupoints of Renzhong （DU 26）, Neiguan （PC 6）, Quchi （LI 11） and Zusanli （ST 36） in rats. In the apurinic/apyrimidinic endonuclease DNA basic group reparative excision experiments, the apurinic/apyrimidinic endonuclease activity of brain tissue increased slightly after electroacupuncture in rats. In the DNA polymerase β （Pol β）experiments, the Pol β ac-tivity of brain tissue increased after electroacupuncture, especially at DU 26 and PC 6. In the DNA ligase experiments, the expression of DNA ligase 1 in brain tissue increased significantly after electroacupuncture. These findings demonstrate increased activity of apurinic/apyrimidinic en-donuclease, Pol β and DNA ligase 1 after electroacupuncture at DU 26 and PC 6. Also, DNA repair in brain tissue supplied by the middle cerebral artery is promoted after electroacupuncture at DU 26 and PC 6, which are more effective than the LI 11 and ST 36 acupoints .
This study compares the changes of DNA repair in brain tissue dominated by the middle cerebral artery after electroacupuncture at the acupoints of Renzhong （DU 26）, Neiguan （PC 6）, Quchi （LI 11） and Zusanli （ST 36） in rats. In the apurinic/apyrimidinic endonuclease DNA basic group reparative excision experiments, the apurinic/apyrimidinic endonuclease activity of brain tissue increased slightly after electroacupuncture in rats. In the DNA polymerase β （Pol β）experiments, the Pol β ac-tivity of brain tissue increased after electroacupuncture, especially at DU 26 and PC 6. In the DNA ligase experiments, the expression of DNA ligase 1 in brain tissue increased significantly after electroacupuncture. These findings demonstrate increased activity of apurinic/apyrimidinic en-donuclease, Pol β and DNA ligase 1 after electroacupuncture at DU 26 and PC 6. Also, DNA repair in brain tissue supplied by the middle cerebral artery is promoted after electroacupuncture at DU 26 and PC 6, which are more effective than the LI 11 and ST 36 acupoints .
基金
the National Basic Research Program of China (973 Program), No. 2009CB522904
the National Natural Science Foundation of China, No. 81173326, 81072868, 30973802 and 81102661
Supported by Open Research Fund of Zhejiang First-foremost Key Subject-Acupuncture & Moxibustion, No. ZTK2010B05
作者简介
Xiaorong Chang, Professor, Doctoral supervisor, the Third Clinical College of Zhejiang Chinese Medical University, Hangzhou 310005, Zhejiang Province, China; Key Subject of Acupuncture, Moxibustion and Tuina, Key Laboratory of Correlativity between Meridian Point and Viscera, Key Laboratory of Biological Information Analysis for Acupuncture and Moxibustion, Hunen University of Chinese Medicine, Changsha 410007, Hunan Province, ChinaCorresponding author：Jie Yan,, - Professor, Doctoral Supervisor, Key Subject of Acupuncture, Moxibustion and Tuina, Key Laboratory of Correlativity between Meridian Point and Viscera, Key Laboratory of Biological Information Analysis for Acupuncture and Moxibustion, Hunan University of Chinese Medicine, Changsha410007, Hunan Province, China yj5381159@yahoo.com.cnCorresponding author： Jianqiao Fang, Professor, Doctoral Supervisor, the Third Clinical College of Zhejiang Chinese Medical University, Hangzhou 310005, Zhejiang Province, China;fangjianqiao@yahoo.com.cn
