摘要
目的观察参附注射液对局灶性脑缺血-再灌注大鼠脑组织中超氧化物歧化酶(SOD)活性及丙二醛(MDA)、血栓素B2(TXB2)、6-酮-前列腺素F1a(6-keto-PGF1a)含量的影响,探讨参附注射液对脑的保护机制。方法清洁级雄性SD大鼠60只,随机分为3组:假手术组(Sham组,n=20)、脑缺血再灌注组(IR组,n=20)、参附注射液预处理组(SFI组,n=20)。采用线栓法制备大鼠大脑中动脉闭塞后再灌注(MCAOR)模型,观察大鼠MCAOR时神经功能状态,TTC染色测脑梗死面积,同时测定血浆中MDA、SOD、TXB2、6-keto-PGF1a的变化。结果参附注射液能有效改善MCAOR大鼠神经功能缺失症状,明显减小梗死灶。IR组与假手术组比较,血清中SOD活性降低,MDA、TXB2含量增加,6-keto-PGF1a含量降低(P<0.05);参附治疗组与IR组比较,SOD活性增高,MDA、TXB2含量下降,6-keto-PGF1a含量增高(P<0.05)。结论参附注射液对脑缺血再灌注损伤引发的自由基损伤有保护作用,并且能够纠正TXB2/6-keto-PGF1a平衡,达到对脑缺血再灌注大鼠的脑保护作用。
Objective To investigate the effect of Shenfu injection on the MDA,SOD,TXB2 and 6-keto-PGF1a after cerebral ischemia/reperfusion injury in rats.Methods Sixty male SD rats were randomly divided into three groups：Sham group（Sham group,n =20）;encephalic ischemia reperfusion group（IR group,n =20） and Shenfu pretreatment group（SFI group,n =20）.Reversible middle cerebral artery occlusion（MCAO） models were produced by intraluminal suture technique.The extent of neurological deficits was evaluated by Longa method;the infarct area of brain was measured by 2,3,5-triphenyl tetrazolium chloride（TTC） staining technique;while the changes of MDA,SOD,TXB2 and 6-keto-PGF1a were detected in plasma.Results Shenfu injection could effectively improve the symptoms of neurological deficits and significantly diminish the infarct areas of brain of MCAO in rats.Compared with sham operation group,MDA,TXB2 increased（P 0.05）,SOD and 6-keto-PGF1a decreased（P 0.05） in IR group.Compared with IR group,MDA,TXB2 decreased（P 0.05）,SOD and 6-keto-PGF1a increased in Shenfu pretreatment group（P 0.05）.Conclusion Shenfu injection has a protective effect on the brain injury induced by ischemia.Its mechanism is correlated with the anti-radical effects.And it can maintain a balance between TXB2 and 6-keto-PGF1a.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2012年第2期124-127,共4页
Journal of China Medical University
基金
四川省科技厅资助项目(2010JY0094)
作者简介
江承平（1967-），男，副主任药师，本科．
通讯作者：吴碧华，E-mail：chengpingjiang@sina．cn
