摘要
目的:建立续断通络胶囊质量标准。方法:采用薄层色谱法对处方中的狗脊、续断、白芍、槲寄生进行了定性鉴别;采用HPLC,以川续断皂苷Ⅵ为评价指标,色谱柱Agilent C18(4.6 mm×250 mm,5μm),流动相乙腈-水(30∶70),检测波长212 nm,流速1.0 mL.min-1进行含量测定。结果:供试品色谱中,在与对照品或对照药材色谱相应位置上显相同颜色的斑点,阴性无干扰。川续断皂苷Ⅵ在0.45～3.6μg线性关系良好(r=0.999 9),平均加样回收率为98.31%,RSD 1.42%。结论:方法简便、专属性强、重复性好,可有效的控制续断通络胶囊的质量。
Objective：To establish a method for quality control of Xuanduan Tongluo capsule.Method：To establish qualitative analysis in teasel,rhizoma cibotii,radix paeoniae rubra and parasitic loranthus.To determine the content of teasel saponin Ⅵ by HPLC,chromatographic column was Agilent-C18 with acetonitrile-water（30∶70）as the mobile phase.The detection wavelength was set at 212 nm and flow rate was 1.0 mL·min-1.Result：Provide to try article color spectrum amid,correspond the spot in same color of location with the collate article or the collate medicine material color spectrum,feminine gender have no jam.Teasel saponin Ⅵ was separated with a good linearity in the sample size ranges of 0.45-3.6 μg,the average recoveries was 98.31%,with RSD 1.42%.Conclusion：The method is simple,specific and reproducible to control the quality of Xuanduan Tongluo Capsule.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第2期96-98,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
2008年北京市科委创新药物研究项目(D0708021330707)
作者简介
袁玲艳，研究生，从事新药研发工作，Tel：010-51218512，E-mail：bjcczyc@sina．com.
[通讯作者]赵永慧，工程师，从事新药研发工作，Tel：010—51218512，E—mail：zhyh760416@163.com.
