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白腐菌锰过氧化物酶对2,2’,4,4’-四溴联苯醚的降解 被引量:7

Degradation of PBDEs by Manganese Peroxidase of White Rot Fungi Phanerochaete chrysosporium
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摘要 多溴联苯醚(PBDEs)是一类在环境中普遍存在的全球性有机污染物,对这类污染物环境行为的研究虽然已有较多报道,但是微生物降解方面尤其是针对单一胞外酶降解的研究还比较少。文章以白腐菌模式菌种Phanerochaete chrysosporium为对象,研究其分泌的胞外酶锰过氧化物酶(MnP)对环境中最常检测到的2,2’,4,4’-四溴联苯醚(BDE47)的好氧降解,考察不同β-环糊精浓度对该酶降解BDE47的影响,并初步探讨了胞内细胞色素P450的作用。结果表明,MnP能有效降解BDE47,在培养15 d后,扣除空白损失,降解率达到70%左右。低浓度和高浓度的环糊精对MnP降解BDE47无显著性影响。胞内细胞色素P450对BDE47的降解没有明显贡献。 This paper describes a laboratory experiment on aerobic degradation of 2,2',4,4'-tetraBDE(BDE47),one of the commonly found congeners of PBDEs(polybrominated diphenyl ethers),by manganese peroxidase(MnP) which is produced by means of agitated culture of Phanerochaete chrysosporium.Influences of β-cyclodextrin of different concentrations on the biodegradation were investigated as well.The experimental result showed that BDE47 could be degraded by MnP with the degradation rate of about 70% through 15 day incubation and very little influence on the degradation of MnP was detected.
出处 《环境科学与技术》 CAS CSCD 北大核心 2012年第1期20-24,共5页 Environmental Science & Technology
基金 国家自然科学基金项目(20977044) 国家“973”项目(2009CB421604)
关键词 四溴联苯醚 锰过氧化物酶 Tween80 Β-环糊精 生物降解 2 2' 4 4'-tetraBDE(BDE47) manganese peroxidase Tween 80 β-cyclodextrin biodegradation
作者简介 作者简介:阮久莉(1987-),女,硕士研究生,主要研究方向为微生物降解PBDEs,(手机)15896452652(电子信箱)ruanjiuli@smail.nju.edu.cn。 通讯作者,教授,博士生导师,研究方向为有机污染物的生物降解,(电话)025-89680359(电子信箱)ecsxg@nju.edu.cn。
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