摘要
采用酶联免疫(ELISA)检测技术,对长江口水样和污水处理厂水样中的典型雌激素雌二醇(E2)进行分析,并与化学检测浓度进行验证比较.结果表明:ELISA的检测限可低至0.5 ng.L-1,试验的变异系数小于30%,具有较高的灵敏性和良好的重复性;ELISA检测长江口采集的水样中E2浓度范围在0.5 ng.L-1以下到0.7 ng.L-1,污水处理厂水样中E2浓度范围在2.5～11.5 ng.L-1,与液相色谱串联质谱(LC-MS/MS)的分析结果相比,相关系数R2为0.9147,表明两种方法具有良好的相关性;ELISA检测所需的水样量比LC-MS/MS更少,使用96微孔板可同时对43个样品进行检测,从而缩短了单个样品的分析时间,具有快速简便和经济适用性.
This study aimed to determine concentrations of the typical estrogen 17/3- estradiol in water samples collected from the Yangtze estuary and wastewater treatment plants using enzyme -linked immunosorbent assay （ELISA）, and illuminated the feasi- bility and suitability of this method verified by chemical measured concentrations. The results showed that the lowest detection limit of ELISA was 0.5 ng· L^-1 and the coefficient of variance for tests was below 30% which indicated high sensitivity and repeat- ability. The E2 concentrations detected by ELISA were at the range of below 0.5 ng· L^-1 to 0.7 ng· L^-1 for samples in the Yan gtze estuary and 2.5 ng· L^-1 to 11.5 ng· L^-1 for samples in wastewater treatment plants, which had a good correlation （ R2 = 0. 9147 ） with the results obtained from LC - MS/MS. By comparing the two detection methods ,the water amounts of sample preparation in ELISA were lower than those in LC - MS/MS, and ELISA method shortened detection time for every sample attributed to simultaneous determination of 43 samples using the 96-well microplates, which indicated that ELISA was a rapid, convenient and economical method for estrogens analysis.
出处
《上海师范大学学报（自然科学版）》
2011年第6期591-596,共6页
Journal of Shanghai Normal University(Natural Sciences)
基金
上海市环保科研项目(沪环科09-21)
关键词
酶联免疫检测
雌激素
快速筛选
雌二醇
enzyme - linked immunosorbent assay
estrogen
rapid screening
17β - estradiol
作者简介
胡双庆（1978-），男，上海市环境科学研究院环境健康与农村环保研究所工程师
