摘要
为提供黄花石斛药材质量标准的依据,采用HPLC法建立黄花石斛药材指纹图谱。色谱条件为Spher-i-5-RP-18色谱柱(4.6mm×250mm,5μm),流动相为甲醇-水,梯度洗脱,流速1.0mL/min,检测波长260nm。采用中华人民共和国药典委员会出版的中药色谱指纹图谱相似度评价系统软件(版本:2004A)进行数据分析,最终建立了黄花石斛的HPLC指纹图谱,并标定了14个共有指纹峰;方法学考察结果符合指纹图谱技术要求,可用于建立黄花石斛药材的指纹图谱。
To provide the basis for quality standards of Dendrobium dixanthum Rchb, the fingerprints of Dendrobium dixanthum Rchb were established by HPLC in the present study. The samples were separated by a Spheri-5-RP-18 column （4.6 mm×250mm,5μm） with methanol-water gradient system as mobile phase. The flow rate was 1.0 mL-min-1 and the detective wave length for the fingerprinting was at 260 nm. Then the chromatograms were compared with ＂Similarity Evaluation System for Chromatographic Fingerprint of TCM＂sofiware published by ChPC （Version 2004A）. Therefore, HPLC fingerprint were established with 14 marked common peaks, and the methodology satisfy the requirements of fingerprint technology. The obtained method was stable and reliable with good repeatability, which may be used to establish HPLC fingerprint of Dendrobium dixanthum Rchb.
出处
《热带农业工程》
2011年第5期1-4,共4页
Tropical Agricultural Engineering
关键词
黄花石斛
指纹图谱
HPLC
Dendrobium dixanthum Rchb
fingerprint
HPLC
作者简介
李维峰（1981-），男，讲师，主要从事药用植物化学成分研究。E-mail：rngcrngc3@gmail.com.
