摘要
采用HPLC法检测猪肉中5种磺胺类药物残留,在NY5029-2001附录E方法的基础上,探讨了流动相、SPE小柱及洗脱流速对试验的影响。结果表明,使用SB C18柱,流动相为甲醇∶乙腈∶2.2%乙酸水溶液=15∶15∶70时,5种磺胺类药物可得到较好的分离,使用Waters碱性氧化铝SPE柱及控制洗脱流速可以得到较高的回收率,提高试验准确性。
Five sulfonamides residues in pork were determined by HPLC.The influences of mobile phase,SPE column and elution rate on the experiment were discussed on the basis of Appendix E,NY5029-2001.Five sulfonamides residues could be separated efficiently when the proportion of methanol to acetonitrile to 2.2% acetic acid solution was 15∶15∶70 with SB C18 column.Considerable recovery and experimental accuracy could be obtained when the Waters alumina B SPE column was employed and the elution rate was controlled.
出处
《中国畜牧兽医》
CAS
北大核心
2011年第12期119-121,共3页
China Animal Husbandry & Veterinary Medicine
作者简介
杨静（1983-），女，河南人，硕士，主要从事畜产品质量安全监测。
