摘要
建立了一种荧光和紫外检测器串联同时测定磺胺类(磺胺甲基嘧啶、磺胺二甲嘧啶、磺胺对甲氧嘧啶、磺胺甲噁唑)和喹诺酮类(诺氟沙星、环丙沙星、恩诺沙星)抗生素的高效液相色谱法.4种磺胺类、3种喹诺酮类抗生素分别在0.50—10.0μg.mL-1、0.01—0.50μg.mL-1范围内线性良好,相关系数R2>0.99.确定了最佳提取液为酸化乙腈,3种喹诺酮类、4种磺胺类药物的检测限(S/N=3)与回收率分别为0.46—0.65μg.kg-1、65.8%—106.3%与0.08—0.19μg.kg-1、90.1%—120.3%.通过对环境样品的检测,发现畜禽废物中磺胺类抗生素的含量高于喹诺酮类,抗生素含量和检出率均较高的药物是环丙沙星、磺胺甲噁唑.猪粪中抗生素的含量高于鸡粪中的,而检出率则低于鸡粪中的.环丙沙星、磺胺甲噁唑可能对农业生态环境安全的威胁更大.该方法简便、快速,可满足畜禽废物中磺胺类、喹诺酮类抗生素的检测.
A reverse-phase high performance liquid chromatographic method detector in series has been established for the simultaneous detection with fluorescence of sulfamerazine, and ultraviolet sulfamethazine, sulfameter, sulfamethoxazole, norfloxacin, ciprofloxacin and enrofloxacin residues in animal waste. The method has good linearity and repeatability, with the correlation coefficient R 〉0.99 between 0.50--10.0 lagμmL-I for the four sulfonamides and 0. 01--0. 50 txg.mL-1 for the three quinolones. The optimum extraction solvent was acid-acetonitrile. The detection limit and recoveries of the three quinolones and four sulfonamides were between 0.46-0.65 txgkg-1 , 65.8%--106.3% and 0. 08-0.19μg.kg-1,90.1%--120.3%. Testing of environmental samples found that the concentration of sulfonamides was higher than that of quinolones in animal waste, with ciprofloxacin and sulfamethoxazole showing high concentration and detection rate. The concentration of the antibiotics in pig manure was higher than that of fowl dung, but the detection rate in fowl dung was higher than that of pig manure. Ciprofloxacin and sulfamethoxazole might have greater influence on agriculture environmental safety. The method was simple, rapid, and could meet the detection of sulfonamides and quinolones in animal waste.
出处
《环境化学》
CAS
CSCD
北大核心
2011年第12期2054-2059,共6页
Environmental Chemistry
基金
广东省农科院科技支撑项目(07-支撑-32)
广东省农科院院长基金项目(20090106)支持
公益性行业(农业)科研专项(3-26)资助
作者简介
通讯联系人，Tel：020-85161473；E—mail：lyaolx@yahoo．com．cn
