摘要
本实验采用薄层色谱法对川楝子中香草酸、异香草酸进行定性鉴别;采用高效液相色谱法对川楝子中香草酸进行含量测定。色谱柱:Hypersil BDS C18(250 mm×4.60 mm,5μm),柱温:30℃,流动相:甲醇∶水∶冰乙酸=25∶75∶0.5,流速:1.0 mL.min-1,检测波长:260 nm。最终,定性鉴别斑点清晰。结果表明香草酸含量在1.022～16.352μg范围内,进样量与峰面积呈良好线性关系,相关系数r=0.9998;香草酸的平均回收率为102.45%,RSD=1.22%(n=6)。
In this research, vaninic acid and iso-vanillic acid in Melia toosendan Sieb. were identified by TLC. The content of vanillic acid was determined by HPLC. The HPLC system consisted of BDS-C18 methanol-water-acetic acid （25 ：75：0.5） as mobile phase,flow rate of 1.0 mL/min and column tempreture of 30 ℃. The eluate was detected at 260 rim. The TLC spots developed were fairly clear. The control content of vanillic acid was within the range of 1. 022 - 16. 352 μg, and there was fine linearship between the sample input and the peak area. The average recovery was 102.45%, r=0.9998 （n=6）,RSD = 1.22%.
出处
《天然产物研究与开发》
CAS
CSCD
2011年第6期1103-1106,共4页
Natural Product Research and Development
作者简介
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