摘要
建立了一种测定猪肉中二甲胺四环素的反相-高效液相色谱(RP-HPLC)分析方法。目标物经EDTA-Mcllvaine提取,用Oasis HLB固相萃取小柱净化,流动相为甲醇-咪唑缓冲溶液,体积比20∶80,荧光检测器检测,外标法定量。平均添加回收率在89.6%～94.0%之间,相对标准偏差(RSD)在3.1%～6.5%之间,检出限为0.01mg/kg。该方法简单,灵敏度高,重现性好。
A high performance liquid chromatographic method for the determination of minocycline in meat was investigated.The samples were extracted with EDTA-Mcllvaine and cleaned up with Oasis HLB SPE and analyzed by HPLC with fluorescence detector.In the method,the mobile phase was imidazole buffer solution and methanol（80∶20）.The relative standard deviation and recoveries were 3.1%～6.5% and 89.6%～94.0%,respectively,and the detection limit was 0.01 mg/kg.The method is precise,simple and convenient.
出处
《分析科学学报》
CAS
CSCD
北大核心
2011年第6期811-813,共3页
Journal of Analytical Science
关键词
二甲胺四环素
高效液相色谱
猪肉
Minocycline
High performance liquid chromatography
Meat
作者简介
通讯作者：李涛，女，硕士，助理研究员，研究方向为食品安全．
