摘要
运用毛细管区带电泳(CZE),以pH=6.59的60mmol/L Na2HPO4-NaH2PO4作为电泳缓冲溶液,测定波长为200nm,还原型谷胱甘肽(GSH)和氧化型谷胱甘肽(GSSG)于5min内达到基线分离。GSH和GSSG的线性范围分别为4～1 000μmol/L和2～1 000μmol/L,相关系数R2值均为0.9999,迁移时间和峰面积的相对标准偏差分别小于0.58%和2.30%,GSH和GSSG的检出限分别为1.66μmol/L和0.86μmol/L,回收率在95.4%～103.3%之间,相对标准偏差均小于3.05%(n=3)。建立的新方法用于市售注射用还原型谷胱甘肽钠的分析,测得注射用还原型谷胱甘肽钠中GSSG含量为0.47%,光学纯度为99.53%。
A technique for the analysis of reduced glutathione sodium for injection by capillary zone electrophoresis（CZE） with diode array detector（DAD） has been reported.Separation of GSH and GSSG was achieved in 5 min using a 60 mmol/L Na2HPO4-NaH2PO4 buffer（pH=6.59）.Linear range of GSH and GSSG were 4～1 000 μmol/L and 2～1 000 μmol/L,and relative standard deviation（RSD） of migration time and peak area were less than 0.58% and 2.30%,respectively.The detection limits were 1.66 mmol/L for GSH and 0.86 mmol/L for GSSG.The recoveries were in the range of 95.4%～103.3%,and the RSD for three independent analysis of each sample was less than 3.05%.The new method has been applied to the analysis of reduced glutathione sodium for injection,and the determination results were 0.47% for GSSG and 99.53% for GSH in reduced glutathione sodium for injection.
出处
《分析科学学报》
CAS
CSCD
北大核心
2011年第6期770-772,共3页
Journal of Analytical Science
关键词
谷胱甘肽
毛细管区带电泳
光学纯度分析
Glutathione
Capillary zone electrophoresis
Optical purity analysis
作者简介
通讯作者：吴惠毅，男，教授，研究方向：毛细管电泳分析在临床实验诊断中的应用
