摘要
目的：为了探讨多抗甲素ＰＡＡ与ＩＬ２ 对骨髓移植（ＢＭＴ） 小鼠免疫功能重建的影响。方法：用致死量照射的Ｂａｌｂｃ小鼠移植同系小鼠骨髓细胞后， 分别给予ＰＡＡ、ＩＬ２ 或ＰＡＡ＋ＩＬ２，３０ ｄ 后检查３ 组ＢＭＴ小鼠免疫功能重建的情况。结果：ＢＭＴ后３０ ｄ，自然恢复小鼠免疫功能十分低下，但ＰＡＡ及ＩＬ２ 用药鼠脾细胞对丝裂原反应， 对ＳＲＢＣ特异的ＰＦＣ数， 对同种异型小鼠脾细胞ＤＴＨ 及ＭＬＲ均明显高于移植对照组， 同时ＩＬ２ 的产生能力也明显增强， 而ＰＡＡ＋ＩＬ２ 组作用又明显强于ＰＡＡ 及ＩＬ２ 单独用药组。结论：ＰＡＡ及ＩＬ２ 均能单独从多方面促进ＢＭＴ后免疫功能重建，ＰＡＡ＋ ＩＬ２ 则从多方面表现出一定的协同效应。
Objective:Toexplorethe effectofPAAandIL 2 enhancedimmunoconstitution ofthelethallyirradiated micefollowingsynge neic bone marrowtransplantation. Methods:Thelethallyirradiated Balbc mice were engrafted withsyngeneic bone marrow cells,then were injected with PAAorIL 2 orPAA+IL 2,for7 days.Onthethirtieth day afterBMT,Theauthorsdetectedtheimmunologicalfunction ofBMT mice.Results:Onthethirtieth day after BMT,theimmunologicalfunctions ofthe BMTmice wereseriously deficient,buttherecovery ofthe immunologicfunctionssuch as proliferation ofsplenocytesto ConAand mixedlymphocytereactionto allogeneiccell,the DTHresponsibilityto C57BLCcells,anti SRBCPFCandthe productionofIL 2 bythesplenocytesinthe micethattreatedsingle with PAAorIL 2 postBMTwere markedly enhancedthanthatofBMTmice,andtherecovery ofimmunologicfunctionswere markedlyacceleratedinthe BMTmicetreated with PAAplusIL 2,when compared withthatofthe BMTmiceinjected with PAAorIL 2 alone.Conclusion:PAAandIL 2 can markedly en hancedimmunoconstitution ofBMTmice when be used alone,furthermore,whilethetwodrugs wereappliedsimutaneously ,thecoordinaterole willbeshown.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1999年第11期490-493,共4页
Chinese Journal of Immunology
关键词
多抗甲素
骨髓移植
免疫重建
IL-2
Poly actin A(PAA) Bone marrow transplantation (BMT) Immunoconstitution
