摘要
研制了一种用于体内成像诊断和光动力学治疗的荧光显微内窥术,可根据探测的荧光信号强度,采用反馈算法快速调节不同成像区域的激发光强度,获取高信噪比、大动态范围的荧光图像,实现交互式荧光诊断与光动力学治疗分析。根据反馈的治疗效果,自适应地连续实时调整光剂量,获得最佳的光动力学治疗结果。激发光强度的动态调整由计算机控制数字微镜器件的开关状态及驻留时间来实现。荧光显微内窥镜的外径尺寸为8mm,工作长度为250.3mm,可装配在腹腔镜管鞘中,适用于体内病灶的显微成像诊断。实验结果表明,显微成像视场为600μm,光学分辨率优于2.2μm,图像动态范围可增强200倍以上,有效改善了荧光微弱区域和荧光饱和区域的图像细节和信噪比,提高了诊断准确性。此技术可进一步扩展为共焦三维成像,实现在体组织细胞学分析。
A kind of fluorescence endomicroscopy which can be used for in-vivo imaging in diagnosis and photodynamic therapy is described. According to the fluorescence signal intensity detected, the fluorescence endomicroscope can adjust the excitation power in different regions rapidly using feedback algorithm, to obtain high signal noise ratio （SNR） and high dynamic range images, and achieve interactive fluorescence diagnosis and photodynamic analysis. It can also adjust the light dose continuously according to the feedback treatment effect, getting the best results of photodynamic therapy. Dynamic adjustment of excitation intensity is realized by the ON-OFF state and the residence time of computer-controlled digital micromirror device. The outer diameter of the fluorescence endomicroscope is 8 mm, working length is 250.3 mm. It can be assembled in the sheath tube of standard laparoscope, and adapted to in vivo microscopic imaging diagnosis of the lesion easily. The experimental results show that the field of view is 600 μm, optical resolution is better than 2.2 μm, and the image dynamic range can be enhanced more than 200 times. The fluorescence endomicroscope can improve the details and SNR of fluorescence saturation regions and fluorescence weak regions, and improve diagnostic accuracy at the same time. This technology can be extended to confocal 3D imaging further, and achieve in vivo histological analysis.
出处
《光学学报》
EI
CAS
CSCD
北大核心
2011年第12期182-187,共6页
Acta Optica Sinica
基金
国家自然科学基金(60808030)
国家科技支撑计划(2011BAI12B06)资助课题
关键词
荧光显微内窥术
数字微镜器件
自适应成像
高动态范围
fluorescence endomicroscopy digital micro-mirror device adaptive imaging high dynamic range
作者简介
冯志锋（1987—），男，硕士研究生，主要从事生物光子学方面的研究。E—mail：fzf1002@163．com
导师简介：王立强（1977-），男，博士，副教授，主要从事生物光子学方面的研究。E—mail：wangliqiang@zju．edu．cn（通信联系人）
