摘要
目的:探讨人喉鳞状细胞癌(LSCC)组织中OPN表达,分析其临床病理学意义,及OPN下调后对喉癌细胞株Hep-2生长和侵袭的生物学行为影响。方法:免疫组织化学SP法检测44例喉鳞状细胞癌及癌旁组织手术切除标本OPN的表达,结合喉癌患者的临床病理学特征进行分析。OPN的RNA干扰慢病毒载体(LV-shOPN)转染Hep-2细胞,蛋白质印迹法检测OPN的表达;MTT法检测Hep-2细胞增殖;Transwell侵袭实验检测Hep-2细胞的侵袭力。结果:44例喉癌和癌旁组织相对正常组织中OPN表达阳性率为75.0%(33例)和13.6%(6例),差异有统计学意义,P=0.000;OPN的表达与颈部淋巴结转移、临床分期、病理组织学分级有关,与性别、年龄、原发部位无相关性;体外构建的LV-shOPN能有效抑制Hep-2细胞中OPN蛋白的表达(P=0.117),抑制Hep-2细胞的增殖(P=0.026)和侵袭力(P=0.135)。结论:OPN可能参与了喉癌的发生、发展和转移,有可能成为判断预后的重要生物学标志;LV-shOPN能够有效抑制Hep-2细胞的生长和侵袭力,可望为喉癌治疗提供一定的理论基础。
OBJECTIVE： To study the OPN expressions and de- tect its clinicopathological value in the largngeal squamous cell carci- noma （LSCC） tissues. To further study the biological effect of OPN down-regulation on the proliferation and invasiveness of laryngeal cancer Hep-2 cells. METHODS： SP immunohistochemical method was used to study the OPN expression on the routinely paraffirr em bedded sections of surgical operated specimens of 44 laryngeal squa- mous cell carcinomas and adjacent tissues. Clinicopathological fea- tures were analysized. Lentivirus vector with RNAi small gene se- quence of OPN （LV-shOPN） was transfected into Hep 2 ceils. OPN expression was detected by Western blotting assay, the proliferation of Hep-2 cells was examined by MTT, transwell assay was used to detect the invasion ability of Hep-2 ceils. RESULTS： The positive rate of OPN protein in 44 cases LSCC and adjacent tissues was 75.0^（33 ca- ses） and 13.60/00 （6 cases） respectively, and the difference of OPN was statistically significant （P = 0. 000 ）. The OPN expression levels were significantly correlated with tumor stage, grade and the presence of lymph node,not correlated with age, gender and tumor primary site. In vitro constructed LV-shOPN effectively inhibited the expression of OPN in Hep-2 cell （P--0. 117） and the proliferation and invasiveness of Hep-2 cells （P=0. 026; P=0. 135）. CONCLUSIONS： OPN expression maybe participate in the development and metastasis of LSCC, and may be im- portant biological markers in reflecting the prognosis of the LSCC. LV shOPN can inhibit the proliferation and invasiveness of Hep-2 cells, which is probably expected to provide a theoretical basis for treatment in largngeal carcinoma.
出处
《中华肿瘤防治杂志》
CAS
2011年第21期1681-1684,共4页
Chinese Journal of Cancer Prevention and Treatment
关键词
唾液糖蛋白
干扰RNA
喉肿瘤
浸润
转移
sialoglycoproteins
RNA interference
laryngeal neoplasms
infiltration
metastasis
作者简介
陈剑秋，女，山东济南人，博士，副主任医师，主要从事头颈肿瘤基础及临床方面的研究工作。E—mail：jianqiuc2008@163．com
【通讯作者简介】郑宏良，男，浙江杭州人，博士，主任医师，教授，博士生导师，主要从事头颈肿瘤及嗓音外科学的临床与研究工作。E—mail：zheng—h12004@163．com
