摘要
棉花是我国重要的经济作物之一,富含棉酚、多糖、单宁等其他干扰物质,因此其DNA的提取和纯化较其他作物更为困难。文中对棉花DNA提取方法进行了讨论,其中SDS法和CTAB法是常用的2种提取方法,而CTAB法及其改良法提取的效果最好。
Cotton was one of important economic crops in China.Because of the rich gossypol,polysaccharides,tannins and other interfering compounds in cotton,the extraction and purification of DNA were much more difficult than other crops.The extraction methods of cotton's genomic DNA were reviewed.In these extraction methods,SDS method and CTAB method were commonly used,and the CTAB method and its modified method resulted in best extraction outcome.
出处
《安徽农业科学》
CAS
北大核心
2011年第32期19684-19685,19688,共3页
Journal of Anhui Agricultural Sciences
基金
中央级公益性科研院所基本科研费(ITBB110604)
关键词
棉花
DNA提取
综述
Cotton
Extraction of DNA
Summary
作者简介
谭燕华(1974-),女,湖南茶陵人,助理研究员,硕士,从事转基因作物环境安全评价的研究,E-mail:Tanyanhua518@163.com。
通讯作者,研究员,从事转基因作物环境安全评价研究。