摘要
用试验证实了Ｃｚａｐｅｋ＇ｓ液体培养基培养棉花黄萎病菌所得病菌培养滤液，能使棉苗发病萎蔫甚至干枯死亡；测试了病菌培养滤液对种子发芽、根的生长、愈伤组织诱导、愈伤组织生长及原生质体活力的影响，抗感不同品种表现出较明显的差异。为病菌培养滤液应用于抗病资源的快速筛选与鉴定、体细胞抗性突变体的筛选等研究提供了可靠的参考资料。
The pathomycete of cotton Vedicillium wilt was inoculated and cultured in Czapek's liquid medium,and the culture filtrate were confirmed to cause seedling wilt and dead.Effectof the filtrate on seed germination,callus induction and growth,viability of protoplast weretested.The results showed that the degree of damage differed between resistant and sensitivecultivars. The possible application of pathomycete culture filtrates on the selection of resistantresources and reststant mutants in vitro was disccused.
出处
《棉花学报》
CSCD
北大核心
1994年第3期184-188,共5页
Cotton Science
基金
国家自然科学基金
国际科学基金
关键词
棉花黄萎病
病菌培养滤液
离休培养物
Verticillium dahliae,culture filtrate,cotton culture in vitro
