摘要
目的对酶联免疫检测血样做NAT检测,分析酶联免疫筛查献血者血样误检的原因。方法对献血者血样进行HBsAg、HCVAb和HIVAb二次ELISA筛查,阴性和阳性血样分别做NAT检测,对HBsAg阴性、HBV DNA阳性献血者做HBV两对半免疫检测和罗氏病毒含量定量测定。结果对二次ELISA筛查及转氨酶和螺旋体检测合格的29 852份血样进行NAT检测,其中15份HBV DNA阳性,漏检率为0.50‰,未发现HCV和HIV RNA阳性。15份HBV DNA阳性献血者乙肝两对半免疫检测,HBsAg均为阴性,7份HBsAb阳性,1份HBeAg阳性,7份HBeAb为阳性,11份HBcAb为阳性。其中1份HBsAg阴性DNA阳性血样HBV罗氏定量为2 520IU/ml,血液存在严重传染可能。对151份二次ELISA阳性血样进行NAT检测,有83份阴性,ELISA的灵敏度为81.93%,特异度为99.72%。结论二次酶联免疫检测法的灵敏度较低,增加抗原、抗体检测和NAT检测可减少漏检。
Objective To determine how many blood samples may be overlooked by ELISA testing.Methods First,double ELISA testing was performed on donor blood samples and NAT testing was performed on samples that were negative and positive for HBsAg,anti-HCV,and anti-HIV according to ELISA.Second,the donors＇ samples were pooled with a HAMILTON STAR sampling processor and nucleic acid was extracted with the EZbead System-32.HBV DNA,HCV RNA,and HIV RNA were amplified and detected with an ABI quantitative Fluorescence PCR instrument.Third,donor samples that were negative for HBsAg but positive for HBV DNA were subjected to double ELISA（HBsAg HBsAb HBeAg HBeAb HBcAb） and a Roche Quantitative Assay.At the same time,sensitivity and specificity were preliminarily determined.Results Of 30 315 donor blood samples,29 852 samples tested with ELISA and negative for ALT and syphilis were tested with NAT;15 samples were positive for HBV DNA.No HCV RNA and HIV RNA were detected.None of the 15 donor samples were positive for HBsAg,7 were positive for HBsAb,1 was positive for HBeAg,7 were positive for HBeAb,and 11 were positive for HBcAb.One result tested positive with 2 520 IU/ml according to the Roche Quantitative Assay.Of 151 donor samples that tested positive according to ELISA,83 tested negative according to NAT testing.Conclusion The results showed that screening with ELISA missed 0.50‰ of HBV with HBsAg.Double ELISA testing had a sensitivity of 81.93% and specificity of 99.72%.
出处
《中国病原生物学杂志》
CSCD
2011年第10期732-735,共4页
Journal of Pathogen Biology
作者简介
赵俊鹏（1985-），男，在读硕士研究生，从事病原生物学研究。Email：345103770@qq．com
【通讯作者】田喜凤，Email：tstianxifeng@163．com
