摘要
目的研究檀香叶提取物的急性毒性和亚急性毒性,对其食用安全性进行评价。方法将昆明小鼠按性别体重随机分组,采用灌胃法,用檀香叶水提取物、95%乙醇提取物进行小鼠急性毒性实验,分别一次灌胃给药37 g/kg体重、38 g/kg体重,观察7 d,测量小鼠体重,脏器质量,并对肝脏和肾脏进行组织切片。用檀香叶醇提物进行小鼠亚急性毒性实验,1日1次灌胃给药25 g/kg体重,持续7 d,观察15 d,测量小鼠体重,脏器质量,并对肝脏和肾脏进行组织切片。结果 :(1)实验结束后,实验组和对照组小白鼠都无死亡;(2)宰杀的实验组小白鼠的各脏器无明显肉眼可见剖检病变;(3)实验组小白鼠的各脏器系数与对照组的差异不显著(P>0.1),组织病理检查未见异常。结论在本实验条件下,按急性毒性分类,檀香叶提取物属无毒级,檀香叶提取物未见明显的亚急性毒性。
To evaluate the security of extracts from leaves of Santalum album L., the acute and sub-acute toxicity experiments were carried out. Kunming mice were divided into several groups randomly according to their gender and weight. For acute toxicity test, one group was given a dose＇ of 37 g/kg of aqueous extracts and another group of Kunming mice was given a dose of 38 g/kg of ethanol extracts from leaves of Santalum album L. by intragastric injection. The acute toxicity was observed in successive 7 days afterwards. The weight of each mouse was recorded. The weight of their major organs was measured, and section of their liver and kidney was made after mice were killed. For sub-acute toxicity test, mice were treated once daily by intragastric injection with ethanol extracts from leaves of Santalum album L. （25 g/kg.d） in successive 7 days. Sub-acute toxicity was observed in 15 days as what did in the acute tests. The results showed that： In the course of the test, mice of the control or test groups didn＇t die; no pathological change of killed mice had been found in their viscera; viscera coefficients of mice had no significant difference（P〉0.1） between the control and test groupsand no obvious histopathologic change was confirmed. It indicated that under this experiment condition, extracts from leaves of Santalum album L. had no acute and apparent sub-acute toxicities.
出处
《食品科技》
CAS
北大核心
2011年第11期179-182,185,共5页
Food Science and Technology
关键词
檀香叶
急性毒性
亚急性毒性
组织病理检查
老鼠
leaves of Santalum album L.
acute toxicity
sub-acute toxicity
histopathologic examination
mice
作者简介
作者简介：黄娟娟（1984～），女，硕士，研究方向为食品质量与安全。
通讯作者
