摘要
以阿维链霉菌(Streptomyces avermitilis)76-12为出发菌株,采用亚硝基胍、吖啶橙、紫外线和氯化锂分别对其孢子和原生质体进行诱变,经抗代谢物理性筛选,获得一系列高产突变株,其中N-1-2高产突变株的发酵单位是出发菌株的2.47倍。实验中同时获得了只产阿维菌素a组分的突变株G-32、Bla组分含量高的Ave8菌株和产蓝绿色孢子的突变株UA-G等。
Spores and protoplasts of the original strain Streptomyces avermitilis 76 12 were treated with nitrosoguanidine （NTG）, acridine orange, ultraviolet radiation （UV） and LiCl respectively. Then a series of high yield avermectin producing and stable mutants were obtained through mutation and rational selection. The avermection productivity of the mutant N 1 2 was 2.47 times of the original strain. Furthermore, several special mutants were obtained. Mutant strain G 32 produced only ＂a＂ avermectins; mutant strain Ave8 had higher lever of avermectin B1a content than original strain; and mutant strain UA G developed bluegreen spores.
出处
《生物技术进展》
2011年第2期135-139,共5页
Current Biotechnology
基金
国家科技支撑计划项目(2006BAD08B02)资助
关键词
阿维菌素
阿维链霉菌
诱变
理性筛选
avermectin
streptomyces avermitilis
mutation
rational screening
作者简介
陈金辉，硕士研究生，从事抗生素发酵研究。
宋渊，教授，主要从事微生物次生代谢研究。Tel：010-62731329；E-mail：syuan@cau．edu．cn
