摘要
[目的]研究蝴蝶干标本DNA的提取方法。[方法]采用5种不同的方法(SDS法、SDS-巯基乙醇-酚法、CTAB法、饱和NaCl法、SDS-巯基乙醇-氯仿法)对蝴蝶干标本DNA进行提取。[结果]SDS-巯基乙醇-酚法、饱和NaCl法和SDS-巯基乙醇-氯仿法适合蝴蝶干标本基因组DNA的提取,并且能成功地应用于蝴蝶线粒体基因COI、CytB和核基因EF-1α的PCR扩增。[结论]SDS-巯基乙醇-酚法、饱和NaCl法和SDS-巯基乙醇-氯仿法可以用于研究蝴蝶干标本DNA的抽提,为蝴蝶的分子系统进化研究奠定了基础。
[Objective]The aim was to study methods for extracting DNA from dried butterfly specimen.[Method]A total of five methods including SDS method,SDS-mercaptoethanol-phenol method,CTAB method,saturated NaCl method,SDS-mercaptoethanol-chloroform methods were employed to extract DNA from dried butterfly specimen.[Result]SDS-mercaptoethanol-phenol method,saturated NaCl method and SDSmercaptoethanol-chloroform method were suitable for genomic DNA extraction from dried butterfly samples,and the DNA extracted can be successfully applied to the PCR amplification of butterfly mitochondrial COI,CytB and nuclear gene EF-1α.[Conclusion]SDS-mercaptoethanolphenol method,saturated NaCl method and SDS-mercaptoethanol-chloroform method can be used to study the extraction of DNA from dried butterfly samples,which laid foundation for study on molecular phylogenetics of butterfly.
出处
《安徽农业科学》
CAS
北大核心
2011年第28期17337-17339,共3页
Journal of Anhui Agricultural Sciences
基金
青海师范大学博士科研启动项目
关键词
蝴蝶:干标本
DNA提取
Butterfly
Dry specimens
DNA extraction
作者简介
乔枫（1973-），女，内蒙古武川人，副教授，博士，从事生物化学与分子生物学研究，E-mail：qiaofnm@163．com。
