摘要
目的研究鲨鱼软骨粉(SCP)对S180荷瘤小鼠免疫细胞功能的影响。方法鲨鱼软骨粉300、200、100mg·kg-1小鼠灌胃给药10 d。MTT法检测其对S180荷瘤小鼠脾淋巴细胞增殖功能的影响;流式细胞仪检测T淋巴细胞亚群的变化;ELISA法测定荷瘤小鼠血清中细胞因子TNF-α、IFN-γ含量。结果 SCP能提高小鼠脾淋巴细胞的增殖活性;不同程度地提高小鼠脾细胞CD4+细胞数,增加CD4+/CD8+细胞比值;明显提高小鼠巨噬细胞的吞噬活性;促进外周血清中TNF-α和IFN-γ的分泌水平。结论 SCP能提高T细胞介导的细胞免疫应答,提高巨噬细胞调节免疫应答的功能,促进TNF-α、IFN-γ等细胞因子的分泌,从而发挥其抗肿瘤作用。
Objective To study the effect of shark cartilage powder（SCP） on immune cells regulatory functions in S180 bearing mice.Methods Shark cartilage powder at doses of 300,200,100 mg·kg-1,ig.for 10 days.The MTT assay was used to determine the effect of SCP on spleen lymphocytes proliferation.The rate of CD＋4/CD＋8 cell was adopted by flow cytometry and the levels of TNF-α and IFN-γ were measured by ELISA method.Results The SCP could increase the proliferation of spleen lymphocytes and the number of CD＋4 cells as well as the ratio of CD＋4/CD＋8 cell significantly.SCP could raise the levels of TNF-α and IFN-γ.Conclusion SCP could enhance the immunologic function,promote the secretions of TNF-α and IFN-γ,and could be applied in anti-tumor therapy.
出处
《中国海洋药物》
CAS
CSCD
2011年第5期29-33,共5页
Chinese Journal of Marine Drugs
基金
黑龙江省自然科学基金项目(D201043)
关键词
鲨鱼
软骨
免疫功能
脾淋巴细胞
细胞因子
shark
cartilage
immune function
spleen lymphocyte
cytokines
作者简介
张秀娟，女，硕士生导师，教授
通讯作者：季宇彬，男，博士生导师，教授E-mail：zxj1717@yahoo．com．cn
