摘要
[目的]优化超声波法提取鸡油菌(Cantharellus cibarius)多糖的工艺,并对其抗氧化性进行研究。[方法]在单因素试验基础上,通过正交试验,对超声波法提取鸡油菌多糖工艺条件进行优化研究,并通过清除1,1-二苯基-2-三硝基苯肼自由基(DPPH.)的试验研究其抗氧化能力。[结果]各因素对多糖得率的影响程度由大到小依次为:超声浸提时间>超声浸提温度=料液比>超声浸提功率。最佳工艺条件为:超声浸提时间2.5 h,超声浸提温度65℃,料液比为1∶40,超声浸提功率800 W,此条件下的多糖得率为4.72%。鸡油菌粗多糖表现出较强的清除DPPH.的能力,其IC50为0.92 mg/ml,鸡油菌多糖具有较强的抗氧化性。[结论]该研究可以为进一步开发利用食药兼用的真菌资源和发挥其潜在的价值提供理论基础。
[Objective] The research aimed to optimize the extraction technology of C.cibarius polysaccharide,and study resistance to oxidation.[Method] The ultrasonic extraction technology of C.cibarius polysaccharide was optimized by orthogonal experiments on the basis of single factor test,and studied the in vitro antioxidant activity of C.cibarius polysaccharide to 1,1-dipheny l-2-pirylhydrazyl（DPPH） free radical.[Result] The experimental results showed that the order of different factors affecting extraction rate of C.cibarius polysaccharide was as follow： Ultrasonic extraction time〉 Ultrasonic extraction temperature= the ratio of material to water〉 Ultrasonic extraction power.The optimal experimental parameters of extracting C.cibarius polysaccharide were as follow： Ultrasonic extraction temperature 65 ℃,the ratio of material to water 1∶ 40,ultrasonic extraction time 2.5 h,ultrasonic extraction power 800 W.Under the optimized conditions,the C.cibarius polysaccharide yield was 4.72%.When its concentration was 0.92 mg/ml,the C.cibarius polysaccharide had a certain ability to scavenge DPPH free radical.[Conclusion] The study can provide theoretical basis for deeply developing and utilizing of fungus resources using in food and medicine and reaching their fullest potential.
出处
《安徽农业科学》
CAS
北大核心
2011年第27期16545-16547,16550,共4页
Journal of Anhui Agricultural Sciences
关键词
鸡油菌
多糖
抗氧化性
C.cibarius
Polysaccharide
Antioxidant activity
作者简介
靳文娟（1986-），女，山西长治人，硕士研究生，研究方向：食品资源研究与开发，E-mail：xykaoyan2007@163．com。
通讯作者，教授，硕士，硕士生导师，从事天然产物研究与开发研究，E-mail：lxxiang@tjcu．edu．cn。
