摘要
采用高效液相色谱-质谱联用法(HPLC-MS)定量检测了白芍药材中的23种农药残留,样品经乙腈提取后再用PSA(N-丙基乙二胺)进行分散固相萃取。方法的定量限(LOQ)为0.75～12.17μg/kg;在0.01和0.1 mg/kg两种添加水平下,除极性较大的噻虫嗪和吡虫啉外,其余农药的回收率均在70%～120%之间,相对标准偏差(RSD)在2.8%～18.9%之间。该方法净化效果好、灵敏度高、重现性好,可用于白芍样品中农药残留的日常检测。
A method of high performance liquid chromatography coupled with mass spectrometry（HPLC-MS） for simultaneous determination of 23 pesticides in traditional Chinese medicine Paeoniae radix Alba was developed.P.radix was extracted with acetonitrile,and then purified using a preliminary sample treatment step based on primary-secondary amine（PSA） dispersive solid-phase extraction.Limits of quantification（LOQ） were between 0.75-12.17 μg/kg.Recovery between 70% and 120% were obtained for all pesticides at 0.01 and 0.1 mg/kg levels,except for polar pesticides such as thiamethoxam and imidacloprid.This method is accurate,reproducible and has good cleanup effect.It could be applied in pesticide residue analysis in P.Radix.
出处
《农药学学报》
CAS
CSCD
北大核心
2011年第5期496-502,共7页
Chinese Journal of Pesticide Science
基金
"重大新药创制"科技重大专项课题(2009ZX09502-024)
关键词
高效液相色谱-质谱联用法
分散固相萃取
白芍
农药
残留
high performance liquid chromatography-mass spectrometry
dispersive solid-phase extraction
Paeoniae radix
pesticide
residues
作者简介
刘银（1988-），女，江西人，硕士研究生，E-mail：20919047@zju．edu．cn
通讯作者（Author for correspondence）：王书芳（1974-），女，河北人，博士，副研究员，主要从事巾药质量控制研究，电话：0571—88208426，E-mail：wangsf@zju．edu．cn
