摘要
为分析和比较白燕与血燕的营养成分,分别采用凯氏定氮法、邻苯二甲醛-氯甲酸芴甲酯(OPA-FMOC)柱前衍生反相高效液相色谱法、柱前衍生二极管阵列/荧光检测器串联反相高效液相色潽法和电感耦合等离子体质谱法(ICP-MS)等方法测定了白燕与血燕的蛋白质含量、氨基酸组成及比例、唾液酸含量、无机元素含量。结果表明:白燕蛋白质含量为56.5%,氨基酸总量49.22%,其中必需氨基酸占氨基酸总量的58.0%,唾液酸含量占干重15.3%。血燕蛋白质含量为55.0%,氨基酸总量44.16%,必需氨基酸占氨基酸总量的57.9%,唾液酸含量约为13.3%。两种燕窝中蛋白质、氨基酸含量均较高,必需氨基酸占氨基酸总量50%以上,唾液酸含量均大于10%,无机元素种类丰富。白燕的蛋白质含量、氨基酸总量、唾液酸含量比血燕略高,但两者不存在明显差异,两种燕窝中无机元素含量略有差异。该结果为燕窝产品的深入研究提供了重要依据和实验思路。
To compare the nutritional components in White-EBN and Red-EBN,protein was determined by using Kjeldahl method,precolumn derivatization with o-phthaldialdehyde （OPA） and fluorenylmethyl chloroformate （FMOC chloride） was used on the determination of amino acids,pre-column derivation reversed phase high performance liquid chromatography with photodiode array detector（DAD） and fluorescence detector（FLD） method was used for the determination of N-acetylneuraminic acid,the content of inorganic elements was studied by using inductive coupled plasma mass spectrometry. The results indicated that the protein content in White-EBN was 56.5%,the total amino acid content of White-EBN was 49.22%,in which,the essential amino acid accounted for 58.0% of total amino acid. The protein content in Red -EBN was 55.0% ,the total amino acid content of White- EBN was 44.16% ,in which,the essential amino acid accounted for 57.9% of total amino acid. There were no significant differences among the protein content,amino acids and N-acetylneuraminic acid,although White-EBN had the higher amount than Red-EBN. They had the same inorganic elements, but their contents differed.
出处
《食品工业科技》
CAS
CSCD
北大核心
2011年第10期414-417,共4页
Science and Technology of Food Industry
基金
国家科技支撑计划(2008BAD94B05)
关键词
燕窝(EBN)
氨基酸
无机元素
唾液酸
edible bird＇s nest
amino acid
inorganic elements
sialic acid
作者简介
作者简介：曹妍（1986～），女，硕士研究生，研究方向：食品科学。
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