摘要
为了解竹子对大气O3浓度升高的生理响应规律及耐受O3胁迫能力的种间差异性,为气候变化背景下的竹林培育应对策略提供理论依据,运用开顶式同化箱(OTCs)开展了5个O3浓度梯度(过滤大气CF、环境大气NF、50、100和150nl·L-1)对毛竹(Phyl-lostachys edulis)和四季竹(Oligostachyum lubricum)叶片光合色素、可溶性蛋白、脂质过氧化和抗氧化系统的影响研究。结果表明:处理90d后,随着O3浓度的升高,毛竹和四季竹叶片叶绿素(Chl)、类胡萝卜素(Car)含量均呈下降趋势,但毛竹的降幅大于四季竹。MDA含量、POD活性呈升高趋势,毛竹的升幅大于四季竹。SOD活性毛竹呈下降趋势,而四季竹表现出先升高后降低的规律。可溶性蛋白含量毛竹和四季竹分别呈升高、降低趋势;与CF相比,低O3浓度处理(≤50nl·L-1)并未对毛竹和四季竹造成明显伤害,而高O3浓度处理(≥100nl·L-1)下毛竹和四季竹叶片光合色素含量和SOD活性极显著降低,MDA含量和POD活性极显著提高,导致毛竹和四季竹叶片光合色素合成受阻,叶片老化加快,膜脂过氧化程度加剧,膜结构和抗氧化系统功能遭到破坏;主成分分析表明,四季竹对O3胁迫的耐受能力明显强于毛竹。
In order to understand the bamboos interspecific differences in physiological responses to elevated atmospheric O3 concentration and in O3 stress tolerance under the background of climatic change,and to provide theoretical evidences for bamboo cultivation,open-top chambers（OTCs） were employed to approach the effects of elevated O3 on the leaf photosynthetic pigment content,soluble protein content,lipid peroxidation,and anti-oxidation system of Phyllostachys edulis and Oligostachyum lubricum.Five treatments were installed,i.e.,filtered air,ambient air,50 nl O3·L-1,100 nl O3·L-1,and 150 nl O3·L-1.After treated with increasing concentration O3 for 90 days,the leaf chlorophyll and carotenoid contents of the two bamboo species were decreasing,with the decrements being larger for Ph.edulis,while the leaf MDA content and POD activity were increasing,with the increments being larger for Ph.edulis than for O.lubricum.The leaf SOD activity of Ph.edulis had a decreasing trend,while that of O.lubricum decreased after an initial increase.The leaf soluble protein content of Ph.edulis had an increasing trend,whereas that of O.lubricum was in adverse.Low concentration（≤50 nl·L-1） O3 had less damage to the two species,but high concentration（≥100 nl·L-1） O3 decreased their leaf photosynthetic pigments content and SOD activity and increased their leaf MDA content and POD activity significantly,resulting in the hindering of photosynthetic pigments synthesis,the accelerated leaf aging,the intensified membrane lipid peroxidation,as well as the destruction of leaf cell membrane structure and of anti-oxidation system functions of the two bamboo species.Principal component analysis showed that O.lubricum had a stronger capability of O3 stress tolerance,as compared to Ph.edulis.
出处
《生态学杂志》
CAS
CSCD
北大核心
2011年第10期2191-2196,共6页
Chinese Journal of Ecology
基金
中央级公益性科研院所基本科研业务费专项(RISF6915)
国家林业公益性行业科研专项(201004008)
浙江省省院合作项目(2010SY01)资助
关键词
毛竹
四季竹
臭氧胁迫
抗性生理
主成分分析
Phyllostachys edulis
Oligostachyum lubricum
ozone stress
resistance physiology
principal component analysis.
作者简介
作者简介庄明浩，男，1985年生，硕士研究生，主要从事竹林生理生态研究。E—mail：zhuangmingha03@163．com
通讯作者E-mail：cslbamboo@126．com
