摘要
本实验采用放射免疫测定技术(RIA)分析了9头屡配不孕母牛和6头正常母牛于发情开始后8天内的血清促黄体素(LH)的含量变化。测定结果表明:屡配不孕母牛的LH排卵峰值低于正常母牛,其峰值分别为2.71±1.00ng/ml和4.70±1.71ng/ml,差异显著(P<0.025)。为了初步验证LH峰值与屡配不孕之间的关系,对20头屡配不孕母牛在发情开始至配种前肌肉注射外源性LH180IU,其配种后60天的受胎率为70％(14/20),而未用LH处理的11头屡配不孕母牛的受胎率为18％(2/11),两者差异极显著(P<0.01)。上述实验结果提示:体内LH排卵峰值降低可能是导致乳牛屡配不孕的重要原因之一。
Serum luteinizing hormone (LH) level of 9 repeat breeders (RB) and 6 normal cows ( NC ) were determined by radioimmunoassay ( RIA ) from oestrus to day 8 of the cycle. The result of the experiment indicated that the LH peak level of RB was lower than that of NC (2.71±1.00 ng/ml vs 4.70 ± 1.71 ng/ml) ( P<0.025 ) . In order to confirm the relationship between scrum LH level and the infertility 20 RB were treated with 180 IU LH ( im ) . The conception rate of these treated RB was 70% ( 14/20 ) , while the untreated group was 18% (2/11) ( P<0.01 ) . The result of the study suggests that low serum LH is very likely to be responsible for the repeat breedings.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
1990年第1期15-19,共5页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
关键词
乳牛
血清LH
屡配不孕
Dairy cow, Serum LH, Repeat breeding
