摘要
以玉米基因组DNA为模板,通过PCR技术扩增得到了腺苷二磷酸葡萄糖焦磷酸化酶AGPase基因编码区上游1 912 bp的启动子(AGPasep)序列。该序列包含TATA-box,CAAT-box等一些高等植物特有的启动子基本核心序列,推断其为一种新的启动子。为了验证该序列是否具有启动子功能,构建了含有此序列的植物表达载体pCAM-AGPasep,通过农杆菌介导法转化玉米愈伤组织进行瞬时表达。GUS染色结果表明,该序列可以驱动GUS基因的表达,具有启动子功能。
Genetic DNA of maize was used as total template to amplify the 1 912 bp promoter upon adenosine diphosphorate glucose pyrophosphorylase AGPase coding region using PCR in this research and named AGPasep.The higher plants basic cored promoter sequences,such as TATA-box and CAAT-box were found among the AGPasep,which is supposed to be a new promoter.To indentify the promoter function of the sequence,we constructed a plant expression vector（pCAM-AGPasep）that was transformed into the maize callus through Agrobacterium-mediated transformation for GUS transient expression.The GUS activity was detected in pCAM-AGPasep-transformed maize callus that indicatd this sequence has the promoter function.
出处
《生物技术通报》
CAS
CSCD
北大核心
2011年第9期72-75,89,共5页
Biotechnology Bulletin
基金
国家转基因生物新品种培育重大专项(2009ZX08003-024B)
作者简介
作者简介：王帅，男，硕士研究生，研究方向：植物分子生物学与基因工程；E-mail：plantscience@yahoo．cn
通讯作者：原亚萍，博士，教授，研究方向：植物分子生物学与基因工程；E—mail：yapingyuan@hotmail．com
